安徽省3种常见蜚蠊的可溶性蛋白质组成分析  

Soluble protein analysis of three ordinary species of cockroaches in Anhui province

作　　者：姚湧[1] 夏立照[1] 

机构地区：[1]安徽医科大学病原生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2001年第2期114-115,共2页Acta Universitatis Medicinalis Anhui

摘　　要：目的　应用十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS PAGE)对安徽省 3种常见蜚蠊的可溶性蛋白质区带进行测定 ,为蜚蠊分类提供科学依据。方法　应用冻融匀浆法制备 3种蜚蠊的可溶性蛋白液 ,进行SDS PAGE ,用考马斯亮蓝G 2 5 0染色后 ,观察、计算各蛋白质区带的分子量。结果　黑胸大蠊的蛋白条带数目♂为 2 9条 ,♀为 31条 ;美洲大蠊的蛋白条带数目♂为 2 8条 ,♀为 30条 ;德国小蠊的蛋白条带数目♂为 2 5条 ,♀为 2 8条。通过比较发现 3种蜚蠊的蛋白质区带数目和位置有差别 ;德国小蠊主要蛋白质区带的位置与两种大蠊相差明显 ;♀♂之间也有差别。结论　SDSObjective To examine the soluble protein patterns of three ordinary species of cockroaches in Anhui province by SDS PAGE technique, and to find the method of scientific classification for cockroaches. Methods The soluble protein liquid was made by the way of freezing and melting homogenate. SDS PAGE was carried out in the liquid. After using Coomassie brilliant blue G 250 , the molecular weight of all of protein strips were measured and calculated by standard curve. Results The numbers of female and male of Periplaneta fuliginosa were 31 strips and 29 strips; the numbers of female and male of Periplaneta Americana were 30 strips and 28strips; the numbers of female and male of Blattella germarica were 28 strips and 25 strips. The number and location of protein bands were different among these species. It was also obvious that the positions of the main protein bands in Blattella germarica were different from those in two Periplaneta species, and the positions of the main protein bands in female insect were different from those in male insect. Conclusion SDS PAGE can be used as one of classification methods for cockroaches.

关 键 词：蜚蠊 蛋白质类 电泳 聚丙烯酰胺凝胶 

分 类 号：Q969.253.1[生物学—昆虫学]