电场作用下超滤粘多糖的膜分子截留特性  

Molecular Retention of Ultrafiltration Membrane for Mucopolysaccharides in the Presence of an Electric Field

作　　者：肖凯军[1] 李琳[1] 郭祀远[1] 陈健[1] 蔡妙颜[1] 

机构地区：[1]华南理工大学食品与生物工程学院轻化工研究所,广州510640

出　　处：《中国海洋药物》2001年第4期7-10,共4页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金项目 (编号 :2 0 0 0 0 60 8);国家教育部科学技术重点项目 (教财司 [1 999]2 84);高等学校博士学科点专项基金项目 (编号 :2 0 0 0 0 5 61 1 7)资助

摘　　要：研究了 90 V/cm电场强度下截留相对分子质量为 10 0 0 0的 PAN膜错流超滤分离粘多糖的分子截留特性 ,采用凝胶过滤色谱法测定超滤透过液中粘多糖相对分子质量分布 ,并利用琼脂糖凝胶电泳法分析超滤分离粘多糖组分和负电性。研究结果表明 :在电场作用下 ,超滤透过液中粘多糖相对分子质量由 170 0 Da增加到 2 2 0 0 0 Da,并能够显著地增加带负电性的酸性粘多糖的膜截留率 。The molecular retention characteristic for mucopolysaccharides through PAN ultrafiltration membrane with a molecular cut-off of 10 000Da under an electric field of 90V/cm electric strength were investigated.The mucopolysaccharides molecular in the permeation solution was measured by gel filtration chromatogaphy and the negatively charged properties of mucopolysaccharides were analyzed by alga gel electrophoresis.These results demonstrated that an electric field can increase the molecular weight distributions of mucopolysaccharides in the permeate from 1 700Da to 22 000Da and the retention of membrane for acid mucopolysaccharides with stronger negative charges,and there fore the neutral mucopolysaccharids selectivel transport through ultrafiltration membrane.

关 键 词：电场 粘多糖 超滤膜 分子截留 鲨软骨粘多糖 

分 类 号：R917[医药卫生—药物分析学]