保留血凝素活性的流行性出血热灭活疫苗免疫家兔的效果  

EFFICACY OF INACTIVATED EPIDEMIC HEMMORRHAGlCFEVER VACCINE WITH 1NTACTHEMOGGLUTININACTIVITY IN RABBITS

作　　者：朱智勇[1] 唐汉英[1] 李岩金[1] 翁景清[1] 付桂明[1] 俞永新[2] 

机构地区：[1]浙江省卫生防疫站 [2]中国药品生物制品检定所

出　　处：《病毒学报》1989年第3期225-229,共5页Chinese Journal of Virology

摘　　要：以流行性出血热(EHF)病毒浙10株为毒种,沙鼠肾原代细胞为基质细胞,β-丙内酯为灭活剂,按类似于流行性乙型脑炎的生物制品规程要求,研制成EHF灭活疫苗。实验证明,此疫苗除了与福尔马林灭活疫苗一样,可用ELISA或反向被动血凝试验(RPHA)检出EHFV抗原外,还可检出较高滴度(64～1024)的血凝素抗原。将此疫苗经肌肉免疫家兔(1ml,2针),在兔血清中不仅能检出荧光(IF)抗体和反向被动血凝抑制(RPHI)抗体,还可测出一定滴度的中和抗体(空斑减少中和试验,简称PRNT,滴度为20～160)和低滴度(10～20)的血凝抑制(HI)抗体。这些结果表明,用β-丙内酯灭活的EHF疫苗与福尔马林灭活的疫苗在免疫原性上有明显区别。用β-丙内酯灭活疫苗免疫的23只家兔,经EHFV浙5株316和3 160ID50的攻击,全部获得了保护。Using the Z10 strain of epidemic hemorrhagic fever ( EHF ) virus as seed, primary kidney cells of Meriones unguiculatus as cell subst rate, β-propiolactone as inactivating agent, an inactivated EHF vaccine was prepared according to a method similar to that of inactivated Japanese B encephalitis vaccine. In this vaccine the EHFV hemagglutinin antigen in higher titres (128-1024) was detected besides the EHFV antigens detected by ELISA or RPHA method which were also present in EHF vaccine inactivated by formalin. Intramuscular immunization of rabbits with this vaccine (1ml twice) produced neutralization (N) antibody (20-160) and hemmagglutination inhibiting(HI)antibody(10-20)besides IF and RPHI antibody, while no HI and little N antibodies were produced by formalin inactivated vaccine. The above results indicate that the immunogenicity of the EHF vaccines prepared with β-propriolactone and with formalin are obviously different. 23 immunized rabbits challenged with EHF virus Z5 strain at 316 or 3160 ID50 showed complete protection.

关 键 词：流行性出血热 灭活疫苗 血凝素 

分 类 号：R373.32[医药卫生—病原生物学]