皮肤晚期反应标本中IL-6和IL-8 mRNA的表达及意义  

Expression and meaning of IL 6 and IL-8 mRNA in late phase reaction of skin

作　　者：吕俊华[1] 熊爱华[1] 孙英[2] 

机构地区：[1]暨南大学医学院,广东广州510632 [2]英国国家心肺研究所

出　　处：《山东医药》2001年第15期1-2,共2页Shandong Medical Journal

摘　　要：为探讨 IL - 6和 IL - 8在皮肤晚期反应 ( L PR)标本中的变化 ,采用原位杂交技术 ,测定了 IL - 6和 IL - 8m RNA在 14例夏季枯草热患者变应原诱导的 L PR标本中的表达。 14例标本中 ,两种细胞因子表达的阳性率分别为 85 .7% ( 12 / 14 )和 92 .8% ( 13 / 14 ) ;与对照标本比较 ,变应原攻击标本中表达上述细胞因子 m RNA的阳性细胞数明显增加 ( P<0 .0 1)。提示 IL- 6和 IL- 8在 L PR的发病中起重要作用。In order to study the change of IL 6 and IL 8 in late phase reaction(LPR)of the skin,the technique of in situ hybridizationwas used to identify messenger RNA for IL 6 and IL 8 in cells infiltrating allergen induced LPR of the skin.Positive rate of the two cytokine expression of 14 samples were 85 7%(12/14)and 92 8%(13/14)respectively.Compared with the diluent sites significantly increased numbers of cells expressing mRNA for IL 6 and IL 8 were observed in the LPR of skin challenged by the allergen(P<0.01).This suggests IL 6 and IL 8 played important role in the process of LPR.

关 键 词：皮肤晚期反应 IL-6 IL-8 CRNA探针 原拉杂交 MRNA 过级反应 

分 类 号：R593.1[医药卫生—内科学] R751[医药卫生—临床医学]