抗丁型肝炎病毒(HDV)抗体酶联免疫测定(ELISA)方法的建立与应用  

ESTABLISHMENT AND APPLICATION OF ELISA FOR DETECTION OF ANTI-HDV ANTIBODY

作　　者：马虹[1] 詹美云[1] 汤少华[1] 张文英[1] 田瑞光[1] 刘崇柏[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1989年第3期263-267,共5页Chinese Journal of Virology

摘　　要：利用大肠杆菌表达的丁型肝炎病毒抗原(HDAg)和从人血清中纯化的抗-HDV IgG,首次在我国建立了检测抗-HDV抗体的ELISA方法。用此法检测53份HBsAg阳性病人和HBsAg携带者血清,并以Abbott Anti-Delta-EIA试剂进行标化,结果完全相同。检测1份抗-HDV抗体阳性血清,其ELISA滴度为80000,高于Abbott EIA试剂检测的ELISA滴度10000。 用此法检测国内部份省市地区HBsAg阳性病人和携带者的血清标本1155份,阳性者同Abbott检测的一致,无假阳性。此法可用于HDV感染的临床诊断,流行病学调查和其它研究,为在我国开展HDV肝炎的研究提供了可靠方法。Anti-HDV ELISA method was established with HDAg expressed in E.coli, purified anti-HDV IgG from human sera, and purified anti-HDV antibody labelled with HRP. This method was used to test anti-HDV antibody in 53 sera collected from HBsAg positive patients and HBsAg carriers. 21 cases were positive. In order to standardize this assay,these samples were also tested by Abbott anti-delta-EIA kit. The results showed that the 100% agreement.The ELISA titer of antibody in one positive serum was 1:80 000 by our ELISA reagent, but 1:10 000 by Abbott EIA kit.This method was used to detect anti-HDV antibody in sera of 1155 HBsAg positive patients and carriers from several provinces and areas of China. The positive rate correspond well with that obtained with Abbott kit. There was no false positive. Since our reagent has high specificity, good sensitivity, is cheap and convenient, it can substitute Abbott kit for clinical daignosis of hepatitis virus infection, epidemiological research and other purposes.

关 键 词：丁型肝炎病毒 ELISA 

分 类 号：R446.61[医药卫生—诊断学]