严重烧伤早期大鼠中性粒细胞、巨噬细胞凋亡实验研究  

An experimental study on the apoptosis of PMNs and macrophages during the early postburn stage in severely scalded rats

作　　者：李志清[1] 黄跃生[1] 杨宗城[1] 

机构地区：[1]第三军医大学西南医院全军烧伤研究所,重庆400038

出　　处：《中华烧伤杂志》2001年第3期171-173,共3页Chinese Journal of Burns

基　　金：国家重点基础研究发展规划项目 (G19990 5 42 0 2 ) ;教育部高等学校骨干教师资助计划

摘　　要：目的　探讨严重烧伤后中性粒细胞 (PMN)凋亡及烧伤血清刺激后PMN、巨噬细胞 (M)凋亡 ,以及巨噬细胞吞噬凋亡PMN的变化。　方法　选用Ⅲ度 30 %TBSA烧伤大鼠模型 ,收集伤后0、6、12、2 4hPMN ,分离伤后 2 4h烧伤血清 ,应用流式细胞技术检测PMN凋亡百分率 ;同时收集大鼠腹腔巨噬细胞 ,检测烧伤血清对M凋亡变化的影响及烧伤后 2 4h大鼠腹腔M对凋亡PMN吞噬能力。　结果　伤后大鼠PMN凋亡百分率明显降低 ,伤后 6、12、2 4h各时相点无显著差异。烧伤大鼠血清刺激 2 4h后 ,PMN凋亡百分率为 (15 4± 1 2 ) % ,较正常大鼠血清刺激后 (5 0 6± 3 9) %显著降低 ;而M凋亡百分率为 (2 3 2± 2 1) % ,较正常大鼠血清刺激后 (11 5± 1 6 ) %增多 ;严重烧伤后大鼠腹腔M对凋亡PMN吞噬能力由 (35 0± 2 6 ) %降至 (2 1 8± 2 1) %。　结论　严重烧伤后及在烧伤血清刺激下大鼠PMN凋亡延迟 ,M吞噬凋亡PMN能力降低 ,可能凋亡的PMN被M吞噬不完全 ,最终坏死 ,释放毒性内容物 ,是引发SIRS的重要因素之一。Objective To investigate the changes of postburn apoptosis of PMN and the apoptosis of PMN and macrophages after being stimulated by burn sera and the change of the phagocytosis of apoptotic PMNs by macrophages. Methods Rats inflicted by 30%TBSA Ⅲ degree scalding were employed as the model. PMNs were harvested on the 0,6,12 and 24 postburn hour(PBH).Burn sera were harvested at 24 PBHs.PMN apoptosis was detected by flow cytometry.Simultaneously,peritoneal macrophages were collected for the study.The apoptotic change of the macrophages(Ms) after being stimulated by burn sera and the ability of Ms phagocytizing apoptotic PMNs at 24 PBHs were examined. Results The PMN apoptosis was obviously inhibited at 6,12 and 24 PBHs in the scalded rats with no difference between the time spots. Twenty four hours after being stimulated by burn sera, the apoptotic rate of PMNs on scalded rats was lower than that in normal rats, while the apoptotic rate of Ms in scalded rats was higher than that in normal rats. The ability of Ms phagocytizing apoptotic PMNs decreased after severe burn(P<0 01). Conclusion There exhibited delayed rat PMN apoptosis and decreased ability of the phagocytosis of apoptotic PMNs by the Ms after severe burn or being stimulated by burn sera. This might lead to the incomplete phagocytosis of apoptotic PMNs by Ms. As a result, the PMNs would finally come to necrosis and release toxic contents which were important factors in the inducing of SIRS. [

关 键 词：烧伤 中性粒细胞 巨噬细胞 凋亡 

分 类 号：R644[医药卫生—外科学]