热休克诱导基因表达谱的变化  

Gene Expression Changes after Heat Shock by cDNA Array Analysis

作　　者：王太宇[1] 吴宁华[1] 沈珝琲[1] 

机构地区：[1]中国医学科学院 中国协和医科大学 基础医学研究所医学分子生物学国家重点实验室,北京100005

出　　处：《中国医学科学院学报》2001年第4期361-364,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金重点项目(39930050)资助

摘　　要：目的 研究细胞在热应激时的基因表达谱变化。方法 提取热休克前后Jurkat细胞的总RNA，经逆转录合成cDNA探针后分别与人cDNA表达点阵杂交，再用ESTblot软件进行分析，并用Northern印迹证明基因表达的改变。结果 经42℃ 1h热休克的细胞中有40个基因的mRNA表达升高，16个基因的mRNA表达降低。其中除热休克基因表达显著增加外，原癌基因c-Jun、cdc 样激酶CLK-1基因表达明显增加。整合素integrin α-4基因，转化生长因子TGF-β基因表达显著降低。Northern 印迹证明c-Jun及hsp90α基因表达升高。结论 在热应激条件下Jurkat细胞热休克基因、c-Jun、CLK-1基因表达增加，integrin α-4基因、TGF-β基因表达减少。Objective To study gene expression changes of cells in response to heat stress, we isolated total RNA from Jurkat cells before and after heat shock treatment． Methods cDNA was labeled with α-3 2P-dATP during reverse transcription of RNA and then used as probe to cDNA expression array． Autoradiogram images were analyzed by ESTblot software． Results After heat shock, the expression level of some forty genes increased while that of sixteen genes decreased． In addition to the elevated expression of heat shock genes, expression of c-Jun and CLK-1 increased most remarkably． The genes with notably decreased expression were integrin α-4 and transforming growth factor β． The elevated expression of c-Jun and hsp90α was further confirmed by Northern blot analysis． Conclusions The expression of some genes in Jurkat cells changes after heat shock treatment． Heat shock induces elevated expression of hsp, c-Jun, CLK-1 gene while decreases expression of integrin α-4 and transforming growth factor β．

关 键 词：人cDNA表达点阵 热休克 基因表达 

分 类 号：R441.9[医药卫生—诊断学]