mm-LDL激活ECV304 BK_(Ca)及丹参与川芎嗪的干预作用  

Interfering Effects of Radix Salviae Miltiorrhizae and Lingustrazine on mm-LDL Activating BK_(Ca) in ECV304 Cell

作　　者：林琳[1] 郑永芳[1] 屈金河[1] 鲍光宏[1] 

机构地区：[1]中国医学科学院 中国协和医科大学 基础医学研究所生理研究室,北京100005

出　　处：《中国医学科学院学报》2001年第4期386-389,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金重大项目(39730220)资助

摘　　要：目的 观察轻度氧化低密度脂蛋白（mm-LDL）对人脐静脉内皮细胞株ECV304的大电导钙激活钾通道（BK_(Ca)）活动的影响，以及丹参(radix salviae miltiorrhizae)水溶提取物764-3和川芎嗪对其效应的干预。方法 细胞贴附式膜片箝技术。结果 mm-LDL(100μg/ml) 可增强ECV304 BK_(Ca)的活动；764-3(30μg/ml)和川芎嗪(200μg/ml)可削弱mm-LDL的激活效应。结论 mm-LDL激活BK_(Ca)的活动，从而增大静息钙内流的电化学驱动力，升高胞内钙浓度，致内皮细胞功能障碍。764-3和川芎嗪通过削弱这一作用而对内皮细胞起到保护作用。Objective To observe the action of minimally modified low density lipoprotein (mm-LDL) on BK_(Ca) in ECV304 cell and the interferance effects of radix salviae miltiorrhizae extract powder 764-3(30μg/ml)and lingustrazine(200μg/ml)on this action． Method The cell-attached configu- ration of patch clamp technique was applied． Results mm-LDL(100 μg/ml) potentiated the activity of BK_(Ca) in ECV304． While 764-3 and lingustrazine abolished it． Conclusions mm-LDL acted on vascular endothelial cell ECV304 could rapidly activate the activity of BK_(Ca) and might result in the increase of electro-chemical gradient for the resting Ca^(2+) influx, thus resting cytoplasmic concentration of calcium could be elevated and endothelial dysfunction would be induced． 764-3 and lingustrazine might have the protective action through decreasing the activity of BK_(Ca)．

关 键 词：钙激活钾通道 轻度氧化低密度脂蛋白 血管内皮细胞 丹参 川芎嗪 

分 类 号：R285.5[医药卫生—中药学]