递呈单纯疱疹病毒糖蛋白C模拟短肽的噬菌体免疫效果的初步研究  

IMMUNOGENICITY OF PHAGES DISPLAYED PEPTIDE MIMICIN GLYCOPROTEIN C OF HERPES SIMPLEX VIRUS

作　　者：杨乔欣[1] 马文煜[1] 余颖[1] 

机构地区：[1]第四军医大学微生物学教研室,西安710032

出　　处：《中国人兽共患病杂志》2001年第4期10-12,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目 (No .39770 0 40 )

摘　　要：目的　鉴定含单纯疱疹病毒 (Herpessimplexvirus ,HSV)糖蛋白C(gC)模拟表位 (Mimotope)的短肽的免疫原性 ,确定其作为新型短肽疫苗组分的可能性。方法　将淘筛噬菌体随机肽库获得的递呈全部或部分 gC模拟肽的噬菌体分为 7组免疫动物 ,ELISA法检测噬菌体免疫效果及天然抗原免疫鼠阳性血清与噬菌体的反应性 ,观察诱生抗体持续时间。结果　递呈 gC模拟肽的噬菌体可诱导小鼠产生抗gC的抗体 ,抗体持续时间超过 4个月 ,用HSV天然抗原免疫小鼠获得的血清抗体也能与各组噬菌体结合。结论　噬菌体递呈肽能够模拟天然HSVgC抗原的部分免疫活性且具有一定的免疫原性 ,可用作HSV亚单位疫苗或表位疫苗的研究。Aim To identify the immunogenicity of oligo-peptides mimicing Herpes simplex virus(HSV) glycoprotein C(gC)and the possibility that the peptides be used as preparative components of novel HSV vaccines.Methods We immunized mice with 7 groups of phages which display entire or part mimic peptides of gC,detected their immunogenicity and reactivity to positive sera immunized with HSV membrane antigen by ELISA.The antibody level was also measured continually.Results The phage displaying mimic peptide of gC could elicits mice antibody directly against HSV gC in the sera,the antibody level was maintained at high level for more than 4 months.The phages derived from peptide library can also specifically react in ELISA with mice sera from individuals immunized with HSV membrane antigen.Conclusion Mimic peptide displayed by positive phages can be used as immunogen instead of the nature antigen for novel HSV vaccine development.

关 键 词：单纯疱疹病毒 噬菌体 表面呈现 免疫效果 

分 类 号：R373.11[医药卫生—病原生物学]