105株流感嗜血杆菌的分离、鉴定与药敏分析  

IDENTIFICATION AND DRUG RESISTANCE OF THE HAEMOPHILUS INFLUENZA

作　　者：彭少华[1] 李从荣[1] 李栋[1] 王婷[1] 

机构地区：[1]武汉大学人民医院,武汉430060

出　　处：《中国人兽共患病杂志》2001年第4期71-72,64,共3页Chinese Journal of Zoonoses

摘　　要：目的　为提高流感嗜血杆菌 (Haemophilusinfluenza ,HIN)的分离率及了解该菌的耐药现状。 方法　配制富含X和V因子的选择性巧克力培养基 ,对 130 8份痰及咽拭标本进行HIN分离、鉴定 ,用头孢硝噻吩法测试 β -内酰胺酶的产生率 ,并用KB法进行药敏试验。结果　呼吸道标本中流感嗜血杆菌的分离率为 8.0 3 % ;HIN的 β -内酰胺酶产酶率为 14.0 %。HIN药敏试验对抗菌药物的敏感率依次为 :阿齐霉素 >头孢呋新 >头孢噻肟 >环丙沙星 >氨苄西林 >头孢他啶 >氯霉素 >复方新诺明。结论　制作富含X和V因子的选择性培养基和床边接种标本能提高HIN的分离率。HIN感染目前可选用阿齐霉素和二代头孢类药物进行治疗 ,临床应关注HIN的耐药性变化。Aim To improve the isolated rate of H influenza(HIN)and investigate the current resistance.Methods Producing selective media with rich Ⅹ and Ⅴ factor.HIN from 1 308 sputa and throat swabs were isolated and identified.The (-lactamases producing rate was examined by nitrocefin.Drug resistance of HIN were examined with K-B method.Results The isolated rate of HIN was 8.03%(105/1 308),(-lactamases producing rate was 14% (7/50).The drug susceptibility in turn was Azithromycin>Cefuroxime>Cefotaxime>Ciprofloxacin>Ampencillin>Ceftazidime>Chloramphenicol>Trimeth/Sulfa.Conclusions The isolated rate of HIN was higher by producing chocolate agar with rich Ⅹ and Ⅴ factor and by-hand culture.Azithromycin and the second cephalosporin can be used for the treatment of HIN infect.

关 键 词：流感嗜血杆菌 分离率 药物敏感试验 

分 类 号：R378.41[医药卫生—病原生物学] R979.4[医药卫生—基础医学]