职业性低浓度烟草尘接触对作业工人淋巴细胞DNA的损伤  

Lymphocyte DNA damage in cigarette factory workers measured by comet assay

作　　者：卢旭[1] 朱昌淇[1] 韦拔雄[1] 刘薇薇[1] 劳向前[1] 陈月华[1] 许启荣[1] 党卫民[2] 林大庆[3] 江朝强[1] 

机构地区：[1]广州市职业病防治院,510620 [2]广东药学院 [3]香港大学社会医学系

出　　处：《中华劳动卫生职业病杂志》2001年第5期351-353,共3页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的　探讨低浓度烟草尘对人体健康的影响。方法　采用单细胞凝胶电泳法检查了10 7名烟草作业工人和 41名非烟草尘暴露的健康成人 (对照组 )的外周血淋巴细胞DNA的损伤。结果　烟草作业工人的彗尾矩 (TM)值为 (4 3.43± 13.77) μm ,高于对照组 [(38.89± 8.98) μm],差异有显著性 (P =0 .0 2 ) ;吸烟也可使TM增加。烟草作业工人中吸烟者TM值为 (5 0 .0 2± 14.82 ) μm ,高于对照组中的吸烟者 [(39.32± 7.12 ) μm],差异有显著性 (P <0 .0 1)。分层分析发现 ,烟草尘暴露虽然可使吸烟者和非吸烟者的TM值均增高 ,但仅吸烟者的TM值与对照组的差异有显著性 (P <0 .0 1) ;同样 ,吸烟引起的TM增高也仅在烟草尘暴露人群中才有显著性 (P <0 .0 0 1)。析因分析也发现 ,烟草职业暴露和吸烟可增加TM值 (P =0 .0 2 5和P =0 .0 0 2 ) ,并且二者有交互作用 (P =0 .0 19)。结论　职业性低浓度烟草尘接触与吸烟可使淋巴细胞DNA损伤增加 。Objective To investigate whether there were separate and combined effects of occupational exposure to tobacco dust and smoking on lymphocyte DNA damage. Method 148 workers(107 workers occupationally exposed to tobacco dust from the production department and 41 controls who were managerial workers) were included in the study.The tail moment(TM) of comet assay was used to measure DNA damage.The two groups had similar mean age,mean duration of work and smoking prevalence. Results The exposed workers had a larger TM than that of the control(mean± s ):(43.43±13.77)μm vs. (38.89±8.98)μm,( P <0.05).Smoking could significantly increases TM[(47.25±14.02)μm vs. (38.90±10.75)μm, P <0.001].Analysis of variance after adjustment for age and gender showed that occupational exposure and smoking had a significant and independent effect on TM( P =0.025 vs. P =0.002,respectively) and there was a significant positive two way interaction between the two factors( P =0.019).Age and gender had no significant effect on TM. Conclusion Smoking and tobacco dust exposure can induce lymphocyte DNA damage and there is a synergistic effect of tobacco dust exposure and smoking on DNA damage.

关 键 词：烟草尘 职业暴露 吸烟 DNA损伤 单细胞凝胶电泳 职业危害 

分 类 号：R135.9[医药卫生—劳动卫生]