疏水作用色谱法同时纯化及复性基因重组人干扰素-α  

Simultaneous Purification and Renaturation of Recombinant Human Interferon-Alpha Expreessed by E.coli by High-Performance Hydrophobic Interaction Chromatography

作　　者：郭立安[1] 

机构地区：[1]解放军第四军医大学化学教研室,陕西西安710032

出　　处：《色谱》2001年第4期301-303,共3页Chinese Journal of Chromatography

基　　金：国家自然科学基金资助项目 (批准号 :30 0 80 0 0 7);高等学校骨干教师计划资助项目 (批准号 :2 0 0 0 65 66)

摘　　要：使用高效疏水作用色谱直接从大肠杆菌表达的基因重组人干扰素 α(rhIFN α)包涵体的裂解液中纯化了rhIFN α ,并在纯化的同时获得了高的复性效率 ,使复性和纯化一步完成 ,大大地简化了操作步骤。用凝胶排阻色谱对该法纯化的rhIFN α进行了纯度测定 ,纯度达到 95 %以上。该法的活性回收率分别比稀释法和透析法高 1 0倍和 1One step simultaneous purification and renaturation of interferon alpha from the inclusion body of E coli expreessed by high performance hydrophobic interaction chromatography(HPHIC) are presented The inclusion body was dissolved by 8 mol/L guanidine hydrochloride The sample lysis was centrifuged and the solution was renaturated by three different methods, i e hydrophobic interaction chromatography, dilution and dialysis The total activity yield renaturation by hydrophobic interaction chromatography was 2 times and 2 6 times as much as that by dilution and by dialysis The purity of the target product was 95% according to high performance size exclusion chromatography and the specific activity was 1 8×10 8 IU/mg The method developed is simpler and more efficient than others

关 键 词：高效液相色谱 纯化 重组蛋白质 基因重组人干扰素-α 高效疏水作用色谱 

分 类 号：TQ464.7[化学工程—制药化工] O657.72[理学—分析化学]