毛细管阵列电泳与规模化DNA测序  

Capillary Array Electrophoresis and High-Throughput DNA Sequencing

作　　者：甄志成[1] 姚志建[1] 

机构地区：[1]国家人类基因组北方研究中心,北京100176

出　　处：《色谱》2001年第4期361-364,共4页Chinese Journal of Chromatography

基　　金：人类基因组计划1%基因组测序项目( 863计划项目 ) (项目批准号 :C1110 0 10 0 198)

摘　　要：根据 10 0 80份基因组DNA测序的结果 ,讨论了毛细管阵列电泳测序方法的技术特点 ,并对影响测序结果的一些因素进行了分析。在此基础上与平板凝胶电泳方法进行比较 ,显示了毛细管阵列电泳的优点。Depending on the outputs of 10?080 DNA sequencing samples from the human genome project, technical features of capillary array electrophoresis are discussed. The results show that 88% of the total readlength were higher than 50 kbp/run. It means that most of the readlength could reach 500 bp or more with reasonable quality. The results were much better than expected when the technology of capillary array appeared about one year ago.\;A key parameter affecting the quality of DNA sequencing is the purity of sequencing template. Therefore, a batch agarose slab electrophoresis was applied to check the purity before loading. Other conditions, such as concentration of template and loading amount, are discussed as well. Finally, comparisons based on the electrophoresis timing, the pre treatment of samples, the loading procedures and the results analysis between capillary array electrophoresis and slab gel electrophoresis have been made. From the point of view of high throughput DNA sequencing, especially in the field of genome research, the capillary array electrophoresis should be the better choice as a more efficient technical platform.

关 键 词：毛细管阵列电泳 DNA测序 基因组 规模化测序 凝胶平板电泳DNA测序仪 

分 类 号：Q75[生物学—分子生物学]