硅胶及其衍生物作为基体的激光解吸离子化飞行时间质谱  

Desorption-ionization Mass Spectrometry with Porous Silica Gel and Its Derivatives as Matrix

作　　者：张清春[1] 邹汉法[1] 郭忠[1] 陈小明[1] 姜泓海[1] 倪坚毅[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心,大连116011

出　　处：《高等学校化学学报》2001年第10期1637-1640,共4页Chemical Journal of Chinese Universities

基　　金：国家杰出青年基金 (批准号 :2 972 5 5 12 )资助

摘　　要：制备了一种新型无机基体材料 (硅胶及其衍生物 )用于测定激光解吸离子化飞行时间质谱 ,建立了一种新的质谱分析方法 ,并用以解决传统 MALDI-TOF MS存在的基体干扰问题 .该法适合于低分子量化合物的分析 .Porous silica gel and its derivatives have been applied as matrix for the analysis of small molecules. In contrast to the conventional MALDI TOF MS, the signal interference of low molecular analytes by the matrix has been eliminated in the MALDI TOF MS with porous silica gel and its derivatives as the matrix. The detection of limit by this method reached the fetomole levels, and almost no fragment of the analytes has been observed. The porous structure of silica gel is essential for effective desorption and ionization of the analytes. Among several different types of silica gel, the silica gel with the pore size of 30 nm gives the best signal. The analyte penetration into the pore is very important. Matrix agents of DHB and CCA have been covalently bound to silica gel for the improvement of the intensity of the spectrum signal.

关 键 词：硅胶 基体 基体固载化 小分子分析 衍生物 质谱分析 激光解吸离子化飞行时间质谱 

分 类 号：O657.63[理学—分析化学]