一种金属螯合连续棒色谱柱的制备及色谱性能  

Preparation and Chromatographic Behavior of Continuous Rod Column for Immobilized Metal Affinity Chromatography

作　　者：卫引茂[1] 黄晓冬[1] 耿信笃[1] 

机构地区：[1]西北大学现代分离科学研究所,西安710069

出　　处：《高等学校化学学报》2001年第10期1664-1666,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :39880 0 0 3);陕西省教育委员会专项科学基金 (批准号 :98JK114 )资助

摘　　要：A procedure for the preparation of continuous rod consisting of poly(glycidyl methacrylate\|co\|ethylene dimethacrylate) for immobilized metal affinity chromatography (IMAC) is presented. When chelating Cu(Ⅱ), Zn(Ⅱ) or Ni(Ⅱ) on it, the rod displayed a property of IMAC and the selectivity for protein separation was different from that obtained from the naked rod. An abnormal increase in retention times of proteins was found under the condition of either very high pH or very low pH on the metal chelated rods.A procedure for the preparation of continuous rod consisting of poly(glycidyl methacrylate\|co\|ethylene dimethacrylate) for immobilized metal affinity chromatography (IMAC) is presented. When chelating Cu(Ⅱ), Zn(Ⅱ) or Ni(Ⅱ) on it, the rod displayed a property of IMAC and the selectivity for protein separation was different from that obtained from the naked rod. An abnormal increase in retention times of proteins was found under the condition of either very high pH or very low pH on the metal chelated rods.

关 键 词：金属螯合色谱 连续棒色谱柱 生物大分子 制备 色谱性能 分离 纯化 

分 类 号：Q503[生物学—生物化学] O657.7[理学—分析化学]