对大骨节病患者体内微小病毒B_(19)核苷酸序列的测定及比较分析  

Detection of HPVB_(19) in Patients with Kashin-Beck Disease and Partial Sequence Comparison

作　　者：陈静宏[1] 王治伦[1] 耿冬[1] 楚雍烈[2] 

机构地区：[1]西安交通大学医学院地方病研究所,陕西西安710061 [2]西安交通大学医学院微生物学教研室,陕西西安710061

出　　处：《地方病通报》2001年第3期1-4,共4页Endemic Diseases Bulletin

基　　金：国家自然科学基金资助项目 (39770 667) ;陕西省科学研究发展计划项目 (96K1 2 G1 2 ) ;陕西省卫生厅科学研究基金项目 (AG1 2 )

摘　　要：对大骨节病患者体内微小病毒 B1 9( HPVB1 9)核苷酸序列进行比较分析 ,利用多聚酶链反应 ( PCR)技术扩增HPVB1 9DNA,将其与 p GEM-载体连接 ,用 T7启动子和 MB反向引物法对 HPVB1 9DNA的 PCR反应片段进行核苷酸序列测定 ,并与 Genebank中一株 HPVB1 9序列 ( AF162 2 7)和文献 [1]报道的一株 HPVB1 9序列进行比较 ,显示 HPVB1 9DNAPCR扩增片段的序列与 AF162 2 7和文献报道的一株 HPVB1 9序列相应片段的同源性分别为 97.75 %、97% ,有点突变和点插入 ,所推测的氨基酸同源性为 93 .5 %差别较大 ,说明大骨节病人微小病毒 B1 9核酸及其氨基酸序列的改变与大骨节病发病有一定相关性。To analyze and compare the nuclear sequence of HPVB 19 in sera of patients with Kashin-Beck Disease, the HPVB 19 DNA was amplified by PCR and linked to pGEM-T vector. The nucleotide sequence was analyzed and compared with one published HPVB 19 nucleotide sequence by Genbank and one published in Journal of virology. The nucleotide sequence homologies between the two published nucleotide sequence were 97.75%, 97%, respectively. The putative amino acid homologies between the two published were 93.5%. The amino acid variation was greater than the nucleotide sequence variation. Results show that there are some relationships beween KBD and the variation of HPVB 19 nucleotide sequence as well as amino acid.

关 键 词：大骨节病 人类微小病毒B19 DNA序列 多聚酶链反应 

分 类 号：R684.1[医药卫生—骨科学]