一种合成多肽——PLNG自由穿膜现象初探  

Cell membrane penetrating property of a novel synthesized peptides——PLNG

作　　者：解军[1] 牛勃[1] 刘红林[1] 张悦红[1] 郭勇[1] 

机构地区：[1]山西医科大学生物化学与分子生物学研究室,太原030001

出　　处：《山西医科大学学报》2001年第4期289-291,共3页Journal of Shanxi Medical University

基　　金：山西省青年科研基金资助项目

摘　　要：目的　研究人工设计合成的多肽PLNG在不同作用环境条件下的跨膜运动现象。方法　体外培养不同组织来源的鼠细胞及CHO细胞、BEL细胞 ,用免疫荧光法观察不同浓度、不同温度、不同反应时间条件下 ,PLNG的穿膜能力及PLNG对不同类型的细胞 (CHO细胞、BEL细胞、成年大鼠肝细胞、幼大鼠肝细胞、成年大鼠心肌细胞、幼大鼠心肌细胞、成年大鼠神经细胞、幼大鼠神经细胞 )的穿膜特性。结果　PLNG在不同作用环境条件下对细胞膜都有穿透作用 ,且进入细胞的量近乎相同。结论　实验观察到PLNG具有广谱的穿膜能力 ,这种穿膜能力在一定范围内对温度、时间及PLNG浓度不敏感 ;而且这种穿膜能力不受组织特异性的限制。Objective To observe the intercellular transport property of a novel synthesized peptide——PLNG. Method Immunofluorescence technique was used to observe the penatration ability and the delivery property of PLNG in different reaction conditions, such as reaction time, reaction temperature, concentration of PLNG, and observe its intercellular delivery property in the medium of different types of cells: adult rat hepatocyte cells, infancy rat hepatocyte cells, adult rat cardiac cells, infancy rat cardiac cells, adult rat nerve cells, infancy rat nerve cells. Results The extensive intercellular delivery property of PLNG was observed in varied condition and types of cells.And there was no significant difference between them. Conclusion It implies that the intercellular property of PLNG is time independent, concentration independent, and energy independent. It is also cell type independent. It suggests that PLNG may be useful in many fields of gene therapy, especially in the field of therapy media in delivery target gene.

关 键 词：肽合成 穿膜肽 跨膜转运 PLNG 

分 类 号：Q516[生物学—生物化学]