免疫器官中肾型鸡传染性支气管炎病毒的定位和动态分布  

Antigen Localization and Distribution of Avian Nephropathogenic Infectious Bronchitis Virus in Inmmune Organs

作　　者：许金俊[1] 陶建平[1] 许益民[1] 万洪全[1] 吴力力[1] 朱坤熹[1] 

机构地区：[1]扬州大学畜牧兽医学院动物医学系,江苏扬州225009

出　　处：《中国预防兽医学报》2001年第6期410-413,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：江苏省教委自然科学基金资助项目

摘　　要：以鼠抗鸡肾型传染性支气管炎病毒 (肾型IBV)的高免血清为一抗 ,辣根过氧化物酶 (HRP)标记的羊抗鼠IgG为二抗 ,建立了检测石蜡切片肾型IBV抗原的间接酶标抗体染色技术。应用该技术对人工感染肾型IBVC90 0 1株鸡免疫器官中的病毒进行了检测 ,结果胸腺和法氏囊是病毒主要侵害的免疫器官 ,脾脏、盲肠扁桃体仅呈一过性感染 ,哈氏腺未检测到。法氏囊从感染后 2～ 12天 ,胸腺从感染后 3～ 8天均检测到病毒抗原 。In this study,an indirect immunoperoxidase technique based on hyperimmune serum was developed to detect avian nephropathogenic infectious bronchitis virus in paraffin_embleded tissue sections of immune organs.The hyperimmune serum was highly specific to IBV T strain,with this technique,antigen of IBV C9001 strain could be detected from 2 days to 12 days in bursal of fabricius,3 days to 8 days in thymus postinfection.Postive staining were mainly observed in lymphocytes and resticulocytes in medulla of bursal follicles and thymic lobules.

关 键 词：免疫器官 鸡 肾型传染性支气管炎病毒 间接酶标抗体染色技术 抗原定位 分布 

分 类 号：S852.659.4[农业科学—基础兽医学] S858.315.3[农业科学—兽医学]