秃杉的组织培养  

Tissue Culture of Taiwania flousiana Gaussen

作　　者：成小飞[1] 李文钿[1] 

机构地区：[1]中国林业科学研究院林业研究所

出　　处：《林业科学研究》1991年第2期147-152,共6页Forest Research

基　　金：林业部攻关课题"珍稀濒危树种的繁殖与保存"

摘　　要：取秃杉吸胀种子的胚、发育10～12天和20天左右的苗端作外植体,进行不定芽诱导,结果表明:不定芽诱导率明显不同,分别为31.4％、80.0％和0。不定芽的发生率主要取决于外植体的发育时期,其次为BA浓度。BA的最适浓度为2.0 mg/L。割取嫩枝插入1/2MS+IBA1.0～2.0mg/L的生根培养基中,生根率可达80％以上。割取嫩枝后的基部转入MS+1.0～2.0mg/L BA+0.5 mg/L IAA的培养基中,不定芽以每周期3～5倍的速度增加。The embryo of imbibitional seed, the apex of 10~12- or 20-day-old seedling (which included cotyledons, shoot apex and 2~3mm hyp-ocotyl stub) were used as explants to induce the adventitious buds. The result showed that the percentage of the induced adventitious buds of the 3 explants were 31.4 %, 80 % and 0 respectively. The proliferation of adventitious bud was firstly depend on the developmental stage of the explant, and secondly on the concentration of N6-benzyl adenine (BA). The optimum concentration of BA for the induction of adventitious bud was 2.0mg/L. The elongated shoots were incubated in 1/2 Murashige and Skoog (MS) medium supplemented with 1.0~2.0mg/L IBA to inducee rooting, 80 % of the shoots were rooted. The residual bases of the shoots were transferred to MS medium in the presence of 1.0~2.0mg/L BA, and 3~5 adventitious buds were regenerated in each base.

关 键 词：秃杉 组织培养 不定芽诱导 增殖 

分 类 号：S791.04[农业科学—林木遗传育种]