HBsAg重组DNA转化中国地鼠卵巢细胞二氢叶酸还原酶阴性突变株的细胞染色体变化及致瘤性  

CHROMOSOME ABERRATION AND CARCINOGENICITY OF CHO(DXB-11) CELLS TRANSFORMED BY PLASMID CONTAINING S GENE OF HEPATITIS B VIRUS

作　　者：田淑芳[1] 任贵方[1] 梅雅芳[1] 王秀萍 王申[1] 朱既明[1] 

机构地区：[1]中国预防医学科学院病毒学研究所

出　　处：《病毒学报》1989年第4期323-326,共4页Chinese Journal of Virology

摘　　要：整合了含乙型肝炎病毒表面抗原(HBsAg)基因和dhfr基因的CHO-dhfr^-细胞,其染色体的畸变率和畸变类型都比亲代CHO-dhfr^-细胞高。但转化前后两系细胞的重要特性都未发生变异,即两者的染色体总数无差别,都是20条。两系细胞株接种裸鼠,均未发现有致瘤性。The frequencies and types of chromosome aberrations induced by integration of plasmid containing of hepatitis B surface antigen and dihydrogen folic acid reductase (dhfr) into CHO-dhfr- (DXB-11) cells were found to be clearly higher when compared with that of the original CHO-dhfr-cells without integrated DNA. There is no change in the number of chromosomes, both cell lines having 20 chromosomes. Both cell lines were non-oncogenic in nude mice.

关 键 词：HBSAG 重组 DNA 染色体畸变 

分 类 号：Q784[生物学—分子生物学]