探讨胎脾LAK细胞对不同的T淋巴白血病细胞的杀伤作用  

Study of the Killing Effect of Fetal Spleen LAK Cells on Different T-_Lymphoid Leukemic Cells

作　　者：张进[1] 聂荣庆[2] 胡国柱[1] 文珠[2] 

机构地区：[1]江西省中医药研究所中心实验室,江西南昌330077 [2]江西省医学科学研究所血液研究室,江西南昌330006

出　　处：《江西医学院学报》2000年第4期17-19,共3页Acta Academiae Medicinae Jiangxi

摘　　要：目的 :探讨胎脾LAK细胞对不同的T淋巴白血病细胞的杀伤作用。方法 :采用低浓度的重组人白介素 2(rhIL 2 )诱导的胎脾LAK细胞杀伤两种不同的T淋巴白血病细胞系MOLT 4和HPB ALL的51Cr释放实验。结果 :发现不同的LAK效应细胞和靶细胞比 (E∶T)条件下 ,胎脾LAK细胞对两种不同的T淋巴白血病细胞杀伤能力随E∶T的增大而增强 (P <0 .0 1)。相同E∶T比时 ,对MOLT 4的杀伤显著强于对HPB ALL的杀伤 (P <0 .0 0 1)。各E∶T比的平均杀伤率MOLT 4为 (5 8.2 4± 12 .93) % ,HPB ALL为 (34.33± 13 .2 0 ) % (P <0 .0 0 1)。结论 :胎脾LAK细胞对不同的T淋巴白血病细胞的杀伤能力存在着差异 ,其可能与T淋巴白血病细胞的分化程度相关。E∶T比越大 。Objective: Study of the killing effect of fetal spleen LAK cells on different T_lymphoid leukemic cells. Methods: The percent rates of 51 Cr_marked T_lymphoid leukemic cells (MOLT_4 and HPB_ALL) killed by fetal spleen LAK cells activated by low concentration recombinant human interleukin_2 (rhIL_2) were detected. Results: The killing ability of fetal spleen LAK cells was positively correlated with the ratio of fetal spleen LAK cells to target cells. By the same ratio of fetal spleen LAK cells to target cells, the killing rate against MOLT_4 was markedly higher than against HPB_ALL (P<0.001). The average killing rates against MOLT_4 and HPB-ALL were (58.24±12.93)% and (34.33±13.20)% (P<0.001). Conclusions: The killing ability of fetal spleen LAK cells is different against different T_lymphoid leukemic cells, and the difference is perhaps derived from different differentiation degree of T_lymphoid leukemic cells.

关 键 词：杀伤细胞 淋巴因子激活 白细胞介素2 白血病 T细胞 对比研究 

分 类 号：R392.12[医药卫生—免疫学] R733.71[医药卫生—基础医学]