魁蚶线粒体16S rRNA和COI基因片段序列测定及其应用前景  

SEQUENCING AND POTENTIAL USES OF MITOCHONDRIAL 16S rRNA AND COI GENE FRAGMENTS IN BLOOD CLAM Scapharca broughtonii

作　　者：孔晓瑜[1] 姜艳艳[1] 相建海[2] 喻子牛[1] 刘亚军[1] 

机构地区：[1]青岛海洋大学教育部海水养殖重点实验室,266003 [2]中国科学院海洋研究所,青岛266071

出　　处：《海洋科学》2001年第12期46-48,共3页Marine Sciences

基　　金：中国科学院实验海洋生物学开放实验室课题资助

摘　　要：Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of partial 16S rRNA gene and partial COI gene were obtained respectively. The contents of A, T, G and C were 24.79%,23.57%,29.16% and 22.48% in 16S rDNA; 22.05%,33.85%,23.33% and 20.77% in COI. The potential uses of these two sequences were discussed for genetic variation, differentiation and relevant research of different geographic populations in the species.Mitochondrial gene(16S rRNA and COI) fragments of Bloody clam Scapharca broughtonii were amplified via PCR, the PCR products were ligated into T vectors, cloned and sequenced. 823 bp and 703 bp nucleotide sequences of partial 16S rRNA gene and partial COI gene were obtained respectively. The contents of A, T, G and C were 24.79%,23.57%,29.16% and 22.48% in 16S rDNA; 22.05%,33.85%,23.33% and 20.77% in COI. The potential uses of these two sequences were discussed for genetic variation, differentiation and relevant research of different geographic populations in the species.

关 键 词：魁蚶 16SrDNA COI 序列测定 线粒体 基因片段 蚶科贝类 

分 类 号：Q959.215[生物学—动物学]