RAPD Analysis of 33 Varieties of Banana  

香蕉33个品种的RAPD研究(英文)

作　　者：杜道林[1] 苏杰[1] 周鹏[2] 郑学勤[2] 黄秉智[3] 李丰年[3] 

机构地区：[1]海南师范学院生物系,海口571158 [2]中国热带农业科学院热带作物生物技术国家重点实验室,海口571101 [3]广东省农业科学院果树研究所,广州510640

出　　处：《Acta Botanica Sinica》2001年第10期1036-1042,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：SupportedbytheMainBioengineeringSubjectofAgricultureMinisterofChina ;EcologySubjectSupportedMainlyinHainanandSubjectoftheEducationDepart mentofHainanProvince;andScientificResearchFoundationofHainanNormalCollege .

摘　　要：RAPD assessment of genetic variations in 33 varieties of banana (Musa nana Lour.) was carried out. Eighteen primers were screened from 249 10 bp arbitrary primers, and a total of 192 DNA bands were amplified, among which 183 (95.31%) were polymorphic. The average number of DNA bands amplified by each primer was 10.67, and the average genetic distance among 33 varieties was 0.3412. Based on UPGMA cluster analysis of 192 DNA bands amplified by 18 primers a DNA molecular dendrogram was established for 33 varieties of banana in China, which divided the 33 varieties into four groups: group A, B, C and D. Group A included 20 varieties, group B included 5 varieties, group C included 2 varieties and group D included 6 varieties. Group A could be divided into 3 sub-groups: A1, A2 and A3. The molecular foundation of genetic diversity of banana was also explored.利用RAPD技术对香蕉 (MusananaLour.) 3 3个品种的遗传变异进行了研究。从 2 4 9个随机引物中筛选出18个有效引物 ,用它们共扩增出 192条DNA带 ,其中 183条为多态性带 ,占 95 .3 1% ,平均每个引物扩增的DNA带数为 10 .67条。利用 18个有效引物扩增的 192条DNA带对香蕉 3 3个品种间的亲缘关系进行UPGMA聚类分析 ,计算出 3 3个品种间的平均遗传距离为 0 .3 4 12。在此基础上建立了香蕉 3 3个品种的DNA分子系统树状图。该系统将香蕉 3 3个品种划归A、B、C和D 4个群 ,其中A群 2 0个品种 ,B群 5个品种 ,C群 2个品种 ,D群 6个品种 ;A群又可以分为 3个亚群。对香蕉遗传多样性分子基础进行了探讨。

关 键 词：BANANA VARIETY RAPD genetic diversity 

分 类 号：S668.101[农业科学—果树学]