不同基模势下luxAB标记的弗氏中华根瘤菌在土壤中的存活能力  

SURVIVAL POTENTIAL OF luxAB MARKED RHIZOBIUM FREDII IN SOIL AT DIFFERENT MATRIC POTENTIAL

作　　者：靳素英[1] 张成刚[1] 田威[2] 仲崇斌[3] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016 [2]沈阳药科大学,沈阳110016 [3]沈阳农业大学,沈阳110161

出　　处：《应用与环境生物学报》2001年第5期465-468,共4页Chinese Journal of Applied and Environmental Biology

基　　金：陆地生态系统生态过程开放实验室;中国科学院沈阳生态试验站基金资助项目

摘　　要：经接合转移将luxAB基因插入弗氏中华根瘤菌 (Rhizobiumfredii)染色体 ,取可高效结瘤固氮的R .frediilux3菌株用于分子生态学研究 .在不同基模势下 ,研究了lux3在土壤中的存活 ,基模势为 - 30和 - 75 0kPa时 ,lux3在灭菌土中的存活能力明显 (P <0 .0 5 )高于未灭菌土 ,当基模势为 - 15 0 0kPa时 ,土壤是否灭菌对lux3的存活影响没有差异 .不同基模势对lux3活性影响显著 ,在 - 15 0 0kPa的未灭菌土中随着饥饿时间的延长 ,可恢复活性的细菌数量显著下降 .比较了测定微生物活性的 3种方法 (即脱氢酶活性、生物发光和微生物呼吸 ) ,发光值变化与活细胞密度变化一致 .图 4表 2参The lux AB gene is a convenient and useful marker for Rhizobium fredii . The lux AB gene was inserted into the chromosome of R. fredii by conjugation. R.fredii lux 3 which could nodulate and fix nitrogen efficiently was used in studying on molecular ecology of R. fredii . The survival and activity of R.fredii lux 3 were studied following inoculation into soil microcosms at different matric potentials. Survival potential of lux 3 was significantly ( P <0.05) greater in sterile than non sterile soil at -30 and -750 kPa, but it was not affected by autoclaving of soil at -1 500 kPa. The activity of R. fredii lux 3 in soil were influenced significantly by different matric potential. Reactivation decreased greatly with prolonged starvation in non sterile soil at -1 500 kPa. Population activity was measured by dehydrogenase activity, bio luminecence and respiration.Metabolic activity measured by luminescence decreased significantly with time ( P <0.05). Fig 4, Tab 2, Ref 8

关 键 词：弗氏中华根瘤菌 luxAB基因 基模势 存活能力 土壤 

分 类 号：S154.381[农业科学—土壤学] Q939.114[农业科学—农业基础科学]