菠菜乙醇酸氧化酶同工酶的亚基组成  

Subunit Components of Glycolate Oxidase Isozymes of Spinacia oleracea

作　　者：徐杰[1] 吴燕燕[2] 

机构地区：[1]华南师范大学生物技术研究所,广州510631 [2]华南师范大学激光生命研究所,广州510631

出　　处：《中国生物化学与分子生物学报》2001年第5期671-674,共4页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金 (No .3 980 0 0 0 9);广东省自然科学基金 (No .0 114 6 9)资助

摘　　要：Protein components of which p I <8.3 and molecular weights 35±2 kD and 25±2 kD may be degradation products of GO isozyme 40±2 kD subunit.But protein band of which p I >8 3 and molecular weight 67±2 kD from the proteins containing GOⅢ and GOⅡ was stable.There was a cross immunoreaction between specific GOⅠ antibody and only 40±2 kD protein band from crude protein of spinach green leaves assayed by SDS PAGE Western blot.Specific antigen from spinach crude protein immunoadsorbed by specific GOⅠ antibody showed four bands with the molecular weights 67±2 kD,40±2 kD,38±2 kD,and 25±2 kD assayed by SDS PAGE, Western blot using the antibody against the protein containing GOⅡ.So 67±2 kD protein could be immunoadsorbed by specific GOⅠ antibody.By native PAGE and SDS PAGE,Western blot using the antibody against the protein containing GOⅡ,rice GOⅡ and a rice 67 kD protein of which p I >8 3 was confirmed to be induced by glycolate substrate.On the results above,a new theory was put forwards that spinach GOⅡ consisted of 67±2 kD and 40±2 kD subunits.Protein components of which p I <8.3 and molecular weights 35±2 kD and 25±2 kD may be degradation products of GO isozyme 40±2 kD subunit.But protein band of which p I >8 3 and molecular weight 67±2 kD from the proteins containing GOⅢ and GOⅡ was stable.There was a cross immunoreaction between specific GOⅠ antibody and only 40±2 kD protein band from crude protein of spinach green leaves assayed by SDS PAGE Western blot.Specific antigen from spinach crude protein immunoadsorbed by specific GOⅠ antibody showed four bands with the molecular weights 67±2 kD,40±2 kD,38±2 kD,and 25±2 kD assayed by SDS PAGE, Western blot using the antibody against the protein containing GOⅡ.So 67±2 kD protein could be immunoadsorbed by specific GOⅠ antibody.By native PAGE and SDS PAGE,Western blot using the antibody against the protein containing GOⅡ,rice GOⅡ and a rice 67 kD protein of which p I >8 3 was confirmed to be induced by glycolate substrate.On the results above,a new theory was put forwards that spinach GOⅡ consisted of 67±2 kD and 40±2 kD subunits.

关 键 词：菠菜 乙醇酸氧化酶同工酶 亚基组成 

分 类 号：Q946.5[生物学—植物学] S636.1[农业科学—蔬菜学]