细胞外信号调节激酶介导单磷酰脂A的心脏保护作用  

Extracellular signal regulatory kinases mediate the cardioprotection induced by monophosphoryl lipid A

作　　者：刘秀华[1] 武旭东[2] 谢晓华[1] 高雪[1] 

机构地区：[1]解放军总医院病理生理学研究室,北京100853 [2]解放军总医院特需医疗部,北京100853

出　　处：《中国病理生理杂志》2001年第10期948-950,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :探讨单磷酰脂A(MLA)的心脏保护作用及其细胞分子机制。方法 :在猪心脏缺血再灌注 (I/R)模型上 ,观察MLA预处理对于心肌梗塞面积的影响。并采用Westernblot的方法 ,检测MAL预处理后猪心肌组织内细胞外信调节激酶 (ERKs)与HSP86含量的变化。结果 :MAL预处理明显缩小猪心脏I/R所致心肌梗塞的范围 ,并使心肌组织内HSP 86和ERKs蛋白水平发生上调。结论 :MLA预处理可以减轻猪心脏I/R所造成的损伤 ,其机制涉及ERKs介导的HSP 86蛋白合成的上调。AIM: To investigate the cardioprotective effects of monophosphoryl lipid A(MLA) and its molecular mechanisms.METHODS: In the ischemia/reperfusion(I/R) model of porcine hearts, the effect of MLA on myocardial infarct size was observed. The protein levels of extracellular signal regulatory kinases (ERKs) and heat shock protein (HSP) 86 were detected by using western blotting method. RESULTS: MLA pretreatment significantly limited the infarct size in the porcine hearts subjected to I/R. It is also found that MLA pretreatment upregulated the protein levels of HSP86 and ERKs. CONCLUSION: MLA pretreatment can attenuate I/R injury in porcine hearts. The protective mechanism might be associated with ERKs-induced upregulation of HSP86 synthesis.

关 键 词：蛋白激酶类 心肌缺血 铁血预处理 心脏保护 细胞外信号调节 单磷酰脂A 

分 类 号：R542.2[医药卫生—心血管疾病]