新生儿脐血淋巴细胞PLCγ1的表达和意义  

The expression and significance of PLCγ1 in the cord blood lymphocytes of neonates

作　　者：何振娟[1] 陈同辛[1] 吴圣楣[1] 应大明[1] 周昕[1] 朱亚忠[1] 

机构地区：[1]上海第二医科大学附属新华医院上海市儿科医学研究所,上海,200092

出　　处：《中国免疫学杂志》2001年第9期503-505,共3页Chinese Journal of Immunology

摘　　要：目的 :探讨新生儿出生时淋巴细胞功能障碍的可能环节。方法 :给予新生儿脐血和成人外周血淋巴细胞抗CD3单克隆抗体、PMA和IM刺激后 ,应用3H TdR掺入法检测淋巴细胞的增殖反应 ;Westernblot法检测淋巴细胞PLCγ1的表达。结果 :抗CD3单克隆抗体刺激 ,脐血淋巴细胞增殖反应明显低于成人 ;PMA协同IM刺激后 ,脐血淋巴细胞的增殖反应明显高于应用抗CD3单抗刺激后的 ;脐血淋巴细胞PLCγ1的表达明显弱于成人。结论 :脐血淋巴细胞功能障碍可能与细胞活化的上游信号转导缺陷有关。Objective:To explore the insufficiency mechanism of the cord blood lymphocytes of the neonates Methods: 3H Thymidine incorporation was used to analyze the proliferative response; Western blot technique was performed to assess PLCγ1 expression between cord and adult lymphocytes Results:The proliferative response of cord blood lymphocytes to anti CD3 mAb was significantly reduced compared to that of the adult's The proliferative response of cord blood lymphocytes to PMA+IM was significantly higher than the response to anti CD3 mAb The PLCγ1 expression was markedly lower than that of the adult's Conclusion:The lymphocytes dysfunction of the neonatal cord blood probably related to the signal transduction in the early stage lymphocytes activation

关 键 词：新生儿 脐带血 淋巴细胞 磷酯酶C PLCγ1 

分 类 号：R722.1[医药卫生—儿科] R392.12[医药卫生—临床医学]