细脚拟青霉多糖Ⅰ的化学结构  

Studies on chemical structure of polysaccharide Ⅰ obtained from Paecilomyces tenuipes

作　　者：陆榕[1] 孙立崧[1] 王仲孚[1] 田庚元[1] 吉田孝[2] 

机构地区：[1]中国科学院上海有机化学研究所生命有机化学国家重点实验室,上海200032 [2]日本国北海道大学理学研究科

出　　处：《中草药》2001年第10期865-867,共3页Chinese Traditional and Herbal Drugs

基　　金：国家攀登计划 ( 970 2 110 0 6 -4 )资助项目

摘　　要：目的　研究细脚拟青霉多糖 的分离纯化方法、相对分子量、单糖组成及其结合方式。方法　室温下水提取出粗多糖 ,采用 Sephadex G- 10 0柱层析纯化。经过酸全水解 ,利用阴离子交换柱测定单糖的组成。甲基化分析测定单糖的结合方式。IR以及 NMR光谱的研究确定糖苷键的类型。结果　经高效液相色谱检测为均一性组成 ,从以后的 GC- MS及 NMR图谱也获得证明。结论　细脚拟青霉多糖 为α- (1→ 6 )连结的葡聚糖 ,相对分子量为 2 .0 5×10 4u。Object To study the isolation and purification of a polysaccharide, obtained from Paecilomyces tenuipes Samson, its molecular weight, sugar composition, and mode of linkage Methods Crude polysaccharide was extracted by water at ambient temperature and purified on Sephadex G 100 column Its monosaccharide composition was determined by ionic ion exchange column after complete hydrolysis with acid Their mode of linkage was determined by methylation and glycosidic linkage established by IR and NMR spectra Results HPLC spectrum showed that the polysaccharide was of homogeneous composition, which was also proved latter by GC MS and NMR Conclusion Polysaccharide obtained from P tenuipes Samson is α (1→6) linked and composed of only D glucose The molecular weight was 2 05×10 4

关 键 词：细脚拟青霉多糖 葡聚糖 甲基化分析 分离 纯化 中药 

分 类 号：R284.1[医药卫生—中药学]