血红素氧化酶-1在离体大鼠心肌缺血预处理中的作用  

Role of heme oxygenase-1 in the ischemic preconditioning of isolated rat heart

作　　者：何洁[1] 张敏[2] 刘俊昌[2] 吴立玲[2] 伍贻经[2] 

机构地区：[1]昆明医学院病理生理学教研室,云南昆明650031 [2]北京医科大学病理生理学教研室,北京100083

出　　处：《中国病理生理杂志》2001年第10期932-934,共3页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目 (No 3 9670 3 0 8)

摘　　要：目的 :观察缺血预处理对离体大鼠心肌缺血再灌注后心功能、乳酸脱氢酶 (LDH)活性和丙二醛(MDA)含量及血红素氧化酶 - 1活性的影响。方法 :应用Langendorff离体大鼠等容收缩灌流模型行离体大鼠心脏灌流。缺血预处理方案为停灌 5min后再灌 5min反复 3次 ,持续缺血再灌方案为停灌 4 0min后再灌 2 0min ,监测正常对照组、缺血再灌组 (IR)和缺血预处理组 (IPC)心功能指标的同时 ,测定冠脉流出液LDH活性、心肌MDA含量和HO- 1活性变化。结果 :IPC组再灌 2 0min时的心功能恢复率显著高于IR组 (P <0 0 1) ,心肌血红素氧化酶 - 1活性也明显高于IR组 (P <0 0 5 ) ,而LDH活性及MDA含量显著少于IR组 (均为P <0 0 1)。结论 :血红素氧化酶 - 1活性增高可能与缺血预处理对大鼠缺血再灌心肌的保护作用有关。AIM: To investigate the influence of ischemic preconditioning on heart function, the activities of lactate dehydrogenase(LDH), malondialdehyde(MDA), and heme oxygenase-1(HO-1) after ischemia/reperfusion in isolated rat heart. METHODS: The model of Langendorff was used in isolated rat heart perfusion. Ischemic preconditioning protocol: stopping perfusion for 5 minutes and reperfusion for 5 minutes, repeating three times. Ischemia protocol: stopping perfusion for 40 minutes and reperfusion for 20 minutes. Indexes of heart function were recorded in control group, ischemia and reperfusion group(IR), and ischemic preconditioning group(IPC). The content of LDH of coronary effluent was measured. Moreover, the content of MDA and activity of HO-1 in myocardium were also measured. RESULTS: The recovery percentage of heart function in IPC group was significantly higher than that in IR group(P<0.01) and the activity of heme oxygenase-1 also increased significantly(P<0.05). CONCLUSION: The contents of LDH and MDA significantly decreased in IPC group compared with IR group. The increase in heme oxygenase-1 activity might be involved in the protective effect of ischemic preconditioning on ischemic/reperfused rat heart.

关 键 词：局部缺血 心肌缺血再灌注损伤 血红素氧化酶-1 离体大鼠 缺血预处理 

分 类 号：R542.2[医药卫生—心血管疾病]