补体C5b-9复合物刺激大鼠肾小球系膜细胞合成NO的机制探讨  

Mechanisms of nitric oxide synthesis induced by human complement C5b-9complex in glomerular mesangial cells of rats

作　　者：王迎伟[1] 何球藻[2] 秦慧莲[2] 高丰光[1] 黄水平[3] 

机构地区：[1]徐州医学院免疫学教研室,江苏徐州221002 [2]复旦大学医学院免疫学教研室,上海200032 [3]徐州医学院统计学教研室,江苏徐州221002

出　　处：《中国病理生理杂志》2001年第10期991-994,共4页Chinese Journal of Pathophysiology

基　　金：江苏省自然科学基金资助项目 (No.BK9715 4 ) ;江苏省卫生厅自然科学基金资助项目 (No .H972 3 )

摘　　要：目的 :探讨人C5b - 9复合物刺激大鼠肾小球系膜细胞 (MC)合成一氧化氮 (NO)的机制。方法 :用人C5b - 9复合物刺激培养的大鼠肾小球MC诱生肿瘤坏死因子α(TNFα)和白细胞介素 1β(IL - 1β) ,并用抗TNFα或抗IL- 1β单克隆抗体进行处理。在上述基础上 ,分析处理 3h、6h及 2 4h时与NO升高有关的某些指标的变化。 结果 :经C5b - 9复合物刺激 6、2 4h后的MC (C5b - 9组 )产生TNFα明显高于对照组 ,并能被TNFα单抗逆转。用C5b - 9复合物刺激MC未见IL - 1β的产生。另用C5b - 9刺激 3h时可见MC表达iNOSmRNA ,而在刺激 6h和 2 4h时 ,MCiNOSmRNA表达 ,MC内cGMP含量及培养上清液中NO-3/NO-2 含量均显著高于对照组。不过 ,C5b - 9刺激时加用TNFα单抗处理 ,这些指标在 6h、2 4h时均较C5b - 9组低。结论 :C5b - 9复合物早期 ( 3h)能诱导MC表达iNOSmRNA ,而 6h后NO的升高则与MC释放的TNFα作用有关。AIM: To explore the mechanism of the nitric oxide (NO) synthesis induced by human C5b-9 complex in glomerular mesangial cells(MC) of rats. METHODS: The MC of rats were cultured and stimulated with human complement C5b-9 complex to induce TNFα and IL-1β. At the same time, several parameters related to NO synthesis were measured at 3 h, 6 h and 24 h after C5b-9 stimulation. The effects of monoclonal antibodies against TNFα and IL-1β on NO synthesis were examined in this system. RESULTS: TNFα concentration in supernatant from MC in C5b-9 group was higher than that of control group at 6, 24 h after stimulation with C5b-9 complex and reversed by adding anti-TNFα McAb. C5b-9 complex didn't stimulate the release of IL-1β in same system. In addition, the expression of iNOS mRNA in MC was observed at 3 h after stimulation with C5b-9. Levels of iNOS mRNA expression and cGMP in MC and NO - 3/NO - 2 in supernatant from MC in C5b-9 group were higher than those in control group at 6, 24 h after C5b-9 stimulation, these changes were also reversed by adding monoclonal antibody against TNFα. CONCLUSION: C5b-9 complex could induce iNOS mRNA expression at 3 h after C5b-9 stimulation, and the synthesis of NO at 6, 24 h was related to TNFα released from cultured MC of rats by C5b-9 complex to a certain extent.

关 键 词：肾小球系膜 一氧化氮 肿瘤坏死因子 白细胞介素1 大鼠 补体C56-9夏合物 mRNA 

分 类 号：R692.6[医药卫生—泌尿科学]