反复低氧对小鼠脑内神经肽Y免疫反应活性的影响  

Effects of repeated hypoxia on the NPY-like immunoreactivity in the mouse brain

作　　者：侯燕芝[1] 焦晓惠[1] 吕国蔚[2] 高翠英[2] 

机构地区：[1]首都医科大学生物化学教研室,北京100054 [2]首都医科大学神经生物学系,北京100054

出　　处：《中国病理生理杂志》2001年第10期1016-1018,共3页Chinese Journal of Pathophysiology

摘　　要：目的 :观察反复低氧对小鼠脑内神经肽Y(NPY)免疫反应活性的影响。方法 :昆明小鼠 4 0只随机分为对照组、低氧 1次 (H1)组、低氧 2次 (H2 )组、低氧 3次 (H3)组和低氧 4次 (H4)组。对低氧各组动物分别行不同次数的反复低氧。各组动物脑内NPY的免疫反应活性采用放射免疫测定法测定。结果 :小鼠对低氧的标准耐受时间随反复低氧次数的增加而显著增加。经 1次低氧和 2次低氧后小鼠脑内NPY的免疫反应活性明显提高 ,分别为正常对照组的 14 5 5 %± 3 2 %和 14 7 3 %± 2 5 % (P <0 0 1) ;而低氧 3次后则降到正常对照水平。结论 :反复低氧通过预适应能显著提高小鼠对低氧的耐受性 ,并可诱导小鼠脑内NPY免疫反应活性在预适应形成的早期一过性地上升。AIM: To observe the effects of repeated hypoxia on the neuropeptide Y(NPY)-like immunoreactivity in the mouse brain. METHODS: Forty male kunming mice were divided into 5 groups: Control, H 1(after the 1 st hypoxic run), H 2(the 2 nd hypoxic run), H 3(the 3 rd hypoxic run)and H 4(the 4 th hypoxic run). The hypoxic groups were subjected to different runs of hypoxia exposure. The NPY-like immunoreactivity in the moue brain was measured by using radioimmunoassary method.RESULTS: The standard tolerance time of the mouse exposed to hypoxia significantly increased following each increase in runs of hypoxia exposure. After the 1 st and 2 nd hypoxic run the NPY-like immunoreactivities in the mice brain significantly increased by 145.5%±3.2% and 147.3%±2.5% compared with the control(P<0.01), respectively. However, the NPY-like immunoreactivites returned to the control levels after the 3 rd run. CONCLUSION: The repeated exposure to hypoxia can significantly enhance mouse's tolerance to hypoxia by preconditioning, and can induce the increase by only one exposure in NPY-like immunoreactivities of the mouse brain during the early period of formation of hypoxic preconditioning.

关 键 词：神经肽Y 低氧 生理学 脑 小鼠 免疫反应 

分 类 号：R338.2[医药卫生—人体生理学] R594.3[医药卫生—基础医学]