pSVPoM cat微基因修饰雪旺细胞移植对损伤脊髓功能恢复的影响  

Microgene pSVPoMcat implanted to modify schwann cells and recovery of function after spinal cord injury

作　　者：陈礼刚 朴永旭[1] 曾凡俊[1] 高立达[2] 毛伯镛[2] 杨立斌[1] 黄茂清[1] 胡威夷[1] 

机构地区：[1]成都军区总医院神经外科,四川成都610083 [2]华西医科大学附属一院神经外科,四川成都610041

出　　处：《第四军医大学学报》2001年第17期1562-1565,共4页Journal of the Fourth Military Medical University

摘　　要：目的　观察 p SVPo Mcat微基因修饰雪旺细胞 (SC)移植对损伤脊髓功能恢复的影响 .方法　采用切割法制备脊髓半横断损伤模型 (T8平面 ) ,实验动物随机植入 p SVPo Mcat微基因的 SC组 (A组 )、SC组 (B组 )和明胶海绵组 (C组 ) ,每组 10只 .用皮层体感诱发电位 (CSEP)及图像分析方法比较各组间诱发电位潜伏期、波幅 ,再生轴突计数 .同时 ,行电镜观察及联合行为 (CBS)记分 .结果　 A,B两组潜伏期和波幅呈恢复趋势 ,且 A组已接近正常 ,与 CBS结果一致 .再生轴突计数 A组为 388± 16 3,B组为 10 9± 16 (P<0 .0 1) ,与电镜观察一致 .结论　 p SVPo Mcat微基因修饰 SC移植对脊髓损伤(SCI)AIM To investigate the effect of microgene pSVPoMcat to modify genetically schwann cell (SC) on recovery of function after spinal cord injury (SCI). METHODS Experimental animals were divided into three groups: the group of microgene pSVPoMcat implanted to genetically SC (group A), SC implanted group (group B) and the control group (group C), each group having 10 animals. The cortical somatasensory evoked patentials (CSEP) and combined behavioral score (CBS) were continuous monitored from operation second week to twelfth week. We used image analysis system to compare the latent period and peek amplitudes of CSEP and count the axon number among the different group. At the same time, electron microscope observation was conduccted. RESULTS The peak latency and peak amplitudes of group A, B had a recovery tendency and it was constant with CBS. The nunnber of regrowed axons of the group A and group B were 388±163 and 109±16, respectively (P<0.01), in line with the observation of electron microscope. CONCLUSION We conclude that the microgene pSVPoMcat to modify genetically SC plays a promotion role in recovery of central function after SCI.

关 键 词：基因修饰 雪旺细胞 移植 脊髓损伤 

分 类 号：R651.205[医药卫生—外科学]