脐血干细胞库的建立及其临床应用  

Establishment of cord blood stem cell bank and its clinical application

作　　者：廖灿[1] 刘斌[1] 黄以宁[1] 李焱[1] 赵民[1] 许遵鹏[1] 汤雪薇[1] 辜少玲[1] 谢杏梅[1] 

机构地区：[1]广州市妇婴医院广州脐血库,510180

出　　处：《中华血液学杂志》2001年第8期411-414,共4页Chinese Journal of Hematology

基　　金：广东省科委重点科技攻关课题 ;广东省卫生厅"五个一工程"重大科研项目 (穗卫科学 1996 2 3 ) ;广州市科委重点攻关课题 (96 Z 64

摘　　要：目的　建立无关供者脐血干细胞库 ,用于治疗骨髓衰竭、恶性及非恶性血液病、某些遗传病、重型免疫缺陷等疾病的干细胞移植。方法　采集足月顺产或剖宫产的新生儿脐血 ,以羟乙基淀粉分离红细胞 ,DMSO/LMD冷冻保存。每份脐血常规检测细胞活率、有核细胞计数、祖细胞体外培养、CD3 4+ 细胞检测、HLA配型及病原学检测等。结果　共采集 2 318份脐血 ,合格 12 40份 ,合格率为5 3 5 % ,合格脐血采集的平均体积 (98.3± 2 7.0 )ml,分离后脐血有核细胞为 (1.2± 0 .5 )× 10 9,回收率为(88.90± 9.6 5 ) %。脐血细胞存活率为 (97.0 0± 2 .6 1) %。CFU GM数为 (5 .18± 14.35 )× 10 5,BFU E数为 (10 .35± 11.5 5 )× 10 5,CFU Mix数为 (1.0 3± 1.47)× 10 5。脐血分离后CD3 4+ 细胞百分率为 (0 .5±0 32 ) % ,细菌培养阳性 4例 ,4例脐血CMV IgM阳性。在广州脐血库的 172例脐血移植登记患者中 ,HLA匹配 4个位点以上的占 91.12 %。至 2 0 0 0年 5月共提供 9份应用于无关供者脐血移植 ,其中 6例植入 ,植入成功率 6 6 .7%。结论　脐血库的建立可使脐血能够较好地应用于造血干细胞移植治疗 。Objective To establish unrelated cord blood stem cell bank for providing potential grafts for stem cell transplantation in the treatment of some malignant and nonmalignant disorders. Methods All cord blood units(CBU) were obtained from full term normal vaginal and Caesarean section deliveries. Most of RBC was removed from CBU by the hydroxyethyl starch (HES) method. CBUs were cryopreserved in liquid nitrogen using DMSO and LMD as cryoprotectants. For each CBU, automated cell count,manual viability,HLA typing, screening for infectious agents,colony forming assays and CD 34 analysis by FACS were routinely performed. Results A total of 2?318 CBUs were collected, among which, 1?240 CBUs met all tests and standards. The average volume of collected qualified cord blood was (98.31±26.95)ml, the average amount of nucleated cells after separation was (1.22±0 47)×10 9, and the average recovery percentage of nucleated cells (88.90±9.65)%. The average viability was (97±2.61)%, the number of CFU GM (5.18±14.35)×10 5, of BFU E (10.35±11.55)×10 5, and of CFU GEMM (1.03±1.47)×10 5. The average percentage of CD 34 + cells after CBU separation was (0.5±0.32)%. Four units were positive in microbiological culture and 4 units CMV IgM positive. In 172 patients registered for cord blood transplantation in Guangzhou Cord Blood Bank, the percentage of 4/6 HLA antigens matched units was 91 12%. Up to May 2000, 9 units had been used in unrelated cord blood stem cell transplantation and 6 of them were successfully engrafted. Conclusion It suggested that cord blood bank could be useful in transplantation and the establishment of cord blood bank had a good prospect.

关 键 词：脐血干细胞库 冷冻保存 临床应用 造血干细胞移植 

分 类 号：R457[医药卫生—治疗学]