绝经对人单核细胞趋化因子受体CXCR2的影响  

EFFECT OF MENOPAUSE ON CHEMOKINE RECEPTOR CXCR2 ON HUMAN MONOCYTE

作　　者：雷著斌[1] 刘建平[1] 魏敏[1] 吕东[1] 

机构地区：[1]济南军区总医院

出　　处：《解放军医学杂志》2001年第11期818-819,共2页Medical Journal of Chinese People's Liberation Army

基　　金：全军医学科研"十五"计划青年基金资助课题 (编号 0 1Q0 15 )

摘　　要：为探讨绝经对雌激素及外周血单核细胞趋化因子受体CXCR2水平的影响 ,选择 2 1例绝经后妇女 ,2 0例绝经前妇女 ,抽空腹静脉血 ,以放免法测定雌激素、明胶法分离外周血单核细胞、流式细胞仪测定单核细胞趋化因子受体CXCR2的蛋白表达和半定量PCR测定其mRNA水平。结果发现 ,绝经后妇女雌激素水平下降 ,而CXCR2蛋白表达及mRNA水平均升高 ,雌激素水平与CXCR2表达呈负相关。To investigate the effect of menopause on chemokine receptor CXCR2 expression on monocyte, 21 postmenopausal and 20 premenopausal women were enrolled in the present study. Fasting venous blood was collected, and plasma estrogen levels were determined with radio immunoassay. Monocytes were separated from peripheral blood with gelatin method. Protein expression of chemokine receptor was assayed with flow cytometry and mRNA concentration was measured with RT PCR. The results showed that the levels of plasma estrogen decreased, whereas protein and mRNA expression of chemokine receptor CXCR2 increased after menopause. The plasma estrogen levels were negatively correlated with chemokine receptor CXCR2 expression in monocytes. These data suggested that the changes in CXCR2 on monocytes might contribute to,at least partially,the increased risk of developing coronary heart disease associated with estrogen secretory changes.

关 键 词：绝经 雌激素类 单核细胞 受体 CXCR2 冠状动脉疾病 冠心病 

分 类 号：R711.51[医药卫生—妇产科学] R541.4[医药卫生—临床医学]