激光切割植物染色体的研究  

The research to Cutting Chromosome of Plant by Laser

作　　者：曹能[1] 周汝敏[1] 欧家鸣[1] 尚鹤岭[1] 龚会云[1] 宋菲[1] 唐继红[1] 

机构地区：[1]云南师范大学生命科学学院,云南昆明650092

出　　处：《云南师范大学学报（自然科学版）》2001年第5期52-54,共3页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助

摘　　要：文章探索了短脉冲激光与微细玻璃针相结合对黑麦染色体进行切割、分离的技术。该技术具有操作简便 ,节省经费 ,容易掌握 ,能减少染色体 DNA的损伤 ,且可对整个细胞进行分离的优点 ,有利于促进染色体或染色体片段分离技术在进行 DNA扩增 ,建立相应的In this article,it have explored the technique to cutting and separate the target chromosomes of rye from other chromosomes of rye by short impulse laser to combine with tiny needle of glass, the technique is provided with operating easy, economizing the use of funds, having in hands easily and could be reducing the damage of DNA in the chromosomes of rye. there are advantages to separating an whole cell, it is advantageous to promoting the technique of separating the chromosomes or fragments of chromosome put in use of increasing DNA and constructing DNA library ;to promoting the technique popularizing.

关 键 词：激光 玻璃针 染色体 分离技术 切割 植物 

分 类 号：Q943[生物学—植物学]