稀土和氮源对南方红豆杉细胞生长及紫杉醇积累的影响  

Effect of rare earth elements and nitrogen sources on cell growth and taxol accumulation in suspension cultures of Taxus. Chinensis var mairei

作　　者：周吉源[1] 杨礼香[1] 

机构地区：[1]华中师范大学生命科学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2001年第3期331-333,共3页Journal of Central China Normal University：Natural Sciences

摘　　要：研究稀土和氮源在 B5培养基上对南方红豆杉细胞悬浮培养时的影响 .结果表明 :在所研究的 3种稀土元素中 ,1× 1 0 - 5mmol/L的 Yb Cl3,最利于细胞生长和紫杉醇的积累 ,紫杉醇含量达1 .3 46mg/L.氮源总浓度 (包括 NH4 +和 NO3- )为 2 7mmol/L,NH4 +和 NO3-比例为 2 /2 5 ,有利于南方红豆杉细胞生长和紫杉醇的合成 .This paper studied the effects of different rare earth elements and different nitrogen concentrations on cell growth and taxol formation in the suspension cultures of Taxus. Chinensis var mairei. YbCl3 is better than LaCl 3 and TbCl 3 for the suspension cultures. The optimum YbCl 3 concentration was 1×10 -5 mmol/L, and high performance liquid chromatographic analysis of cell suspension culture extracts showed that the taxol produced could be as much as 1.324 6 mg/L. The most favorable nitrogen concentration was 27 mmol/L. The best ratio of ammonium to nitrate was 2/25.

关 键 词：南方红豆杉 悬浮培养 稀土 氮源 细胞生长 紫杉醇积累 

分 类 号：S791.49[农业科学—林木遗传育种]