黄芪总皂甙抗HSV_2的实验研究  

The Experimental Research of Antiherpes Simplex Virus Type-2 Effects of Total Saponins in the Root of Astragalus Membranaceus

作　　者：王志洁[1] 黄铁牛[2] 刘焱文[3] 杨占秋[1] 方学韫[1] 

机构地区：[1]武汉大学医学院病毒所,武汉430071 [2]中南财经政法大学 [3]湖北中医学院

出　　处：《浙江中医学院学报》2001年第5期43-46,共4页Journal of Zhejiang College of Traditional Chinese Medicine

基　　金：湖北省自然科学基金资助 (编号 980 5 90 )

摘　　要：目的 :研究黄芪的抗病毒作用。方法 :以阿昔洛韦 ( ACV)为阳性对照 ,采用病毒所致细胞病变的抑制及空斑减数实验 ,观察黄芪总皂甙抗 HSV-2 333株药效。结果 :在 HEp-2细胞系统中 ,ACV对 HSV-2 333株直接杀灭、感染阻断、增殖抑制的ED50 为 1 6.4 5μg/ml、1 8.62μg/ml、1 0 .85μg/ml;黄芪总皂甙的相应数值为 1 .73μg/ml、2 .71μg/ml、2 .74μg/ml。结论 :黄芪总皂甙对 HSV-2　 333株的治疗指数为 ACV的 2 .31、0 .96、1 .64倍 ,值得开发利用。In order to understand and exploit the antiviral properties of Astragalus membranaceus,we carried out this research.Methods We use ACV as positive control of antiherpes,through plaque reduction assay and inhibitions of cellular pathological effects of herpes simplex virus type-2,observe the effects of antiherpes of total saponins of Astragalus membranaceus.Results In Hep-2 cell system,the median effective doses(ED50) of ACV of direct virucidal activity,the inhibition effects on the multiplication and infection of HSV-2 333 strain are 16 45ug/ml,18 62 ug/ml,2 7ug/ml,2 73ug/ml separately.Conclusion The treatment indices(TI) of total saponins to HSV-2 333 strain are 2 31,0 96,1 64 times of ACV Tis,it should be worth exploitation.

关 键 词：黄芪总皂甙 抗病毒 人疱疹病毒 阿昔洛韦 ACV HSV2 实验 比较 

分 类 号：R285.5[医药卫生—中药学]