SHV型β-内酰胺酶的动力学研究  

Study on kinetics of SHV type β-lactamaese

作　　者：谢友红[1] 钱元恕[2] 刘宏[3] 向晓琴[3] 兰雁飞[3] 易刚[3] 许炜[3] 

机构地区：[1]深圳市东湖医院,深圳518020 [2]汕头大学医学院,汕头515031 [3]重庆医科大学附属第一医院,重庆400016

出　　处：《中国抗生素杂志》2001年第4期261-262,289,共3页Chinese Journal of Antibiotics

摘　　要：采用分光光度法研究革兰氏阴性菌产生的 4种 SHV型β-内酰胺酶的动力学。以 L - B作图法求取 Km和 Vmax。除 SHV- 1对第三代头孢菌素头孢噻肟没有水解作用外 ,SHV型β-内酰胺酶对青霉素类、第一代、第二代、第三代头孢菌素均有不同程度水解作用。头孢噻肟对 SHV- 2、SHV- 4、SHV- 5有较高的亲和力(低 Km) ,但 Vmax均不超过 15 % ,SHV型酶的最适 p H值为 6 .6～ 7.4;最适反应温度是 34～ 40℃。The enzyme kinetics of 4 SHV types β lactamases from gram negative bacteria were studied spectrophotometrically. Michaelis constant (Km) and maximal velocity (V max ) were obtained from L B plot, the penicillins, the first, second and third generation cephalosporins were hydrolyzed by SHV type β lactamases in different levels except cefotaxime, which was not hydrolyzed by SHV 1 enzyme. Cefotaxime has demons trated to have a high affinity to SHV 2, SHV 4, SHV 5, but V max to SHV 2, SHV 4 and SHV 5 were less than 15%. The optimal pH of SHV type enzyme was 6.6~7.4, and the optimal temperature was in the range of 34~40℃.

关 键 词：酶动力学 Β-内酰胺酶 Β-内酰胺抗生素 分光光度法 SHV型 

分 类 号：R978.11[医药卫生—药品] R969.1[医药卫生—药学]