蝎毒抗癌多肽及其分离组分分析及鉴定方法  

Research on analysis of APBMV and its components

作　　者：孔天翰[1] 郭进武[1] 陈蕴 韩雪飞[1] 陈华艳[1] 吴逸明[3] 董伟华[1] 

机构地区：[1]河南医科大学生物毒素中心,郑州450052 [2]河南省中药研究所,郑州450004 [3]河南医科大学公共卫生学院,郑州450052

出　　处：《河南医科大学学报》2001年第6期647-650,共4页Journal of Henan Medical University

基　　金：河南省科技攻关资助项目 95 12 0 0 10 2

摘　　要：目的 :探讨蝎毒抗癌多肽 (antineoplasticpolypeptidefromButhusmartensiivenom ,APBMV)及其分离组分的分析及鉴定方法。方法 :采用SepharoseFF阳离子交换凝胶柱 (2 5cm× 5cm交换柱 ,流速 0 .8ml/min ,梯度洗脱 12 0 0min)层析法分离APBMV ,用高效毛细管电泳 (highperformancecapillaryelectrophoresis,HPCE)及高效液相色谱法 (highperformanceliquidchromatography ,HPLC)分析检测。结果 :用SepharoseFF阳离子交换凝胶层析可将APBMV分离为 2个峰。经HPCE、HPLC分析显示 ,APBMV主要成分为峰Ⅰ～Ⅳ ,其分离组分分别以峰Ⅰ或峰Ⅲ为主 ,其纯度分别为86 .35 %、6 9.5 7%。结论 :SepharoseFF用于进一步分离APBMV可望获得较高纯度的单一有效成分 ,HPCE及HPLC均可较好地显示蝎毒中小分子多肽的组成及相对含量 。Aim: To explore the methods on analysis and evaluation of antineoplastic polypeptide from Buthus martensii venom(APBMV) and its components. Methods: The components of APBMV were separated by ion exchange chromatography (Sepharose FF, column:25 cm×5 cm,flow rate:0.8 ml/min,linear gradient:1 200 min),and were inspected by high performance liquid chromatography(HPLC) and high performance capillary electrophoresis(HPCE). Results: APBMV could be separated into 2 components(component 1 and 2 ) by Sepharose FF ion exchange chromatography and 4 peaks(peakⅠ～Ⅳ) by HPLC. It was displayed by HPLC and HPCE that the peakⅠwas mainly made up from the component 1(purification rate: 86.35%) and the peak Ⅲ, the component 2 ( 69.57%). Conclusion: Sepharose FF chromatography is useful for improving the purification rate and output of components of APBMV. The quantity and purity of components from scorpion venom can be displayed obviously by HPLC and HPCE.

关 键 词：蝎毒 抗癌多肽分离 高效液相色谱法 高效毛细管电泳法 APBMV 

分 类 号：R979.1[医药卫生—药品] R917[医药卫生—药学]