普乐可复对肾脏毒性的实验研究  

Experimental study of prograf to nephrotoxicity

作　　者：李中凌[1] 杨锦建[1] 宋东奎[1] 张卫星[1] 

机构地区：[1]河南医科大学第一附属医院泌尿外科,郑州450052

出　　处：《河南医科大学学报》2001年第6期728-730,共3页Journal of Henan Medical University

摘　　要：目的 :探讨普乐可复 (FK5 0 6 )对肾脏的毒性及其机制。方法 :分别给小鼠腹腔注射生理盐水、FK5 0 6 5mg·(kg·d) -1和 10mg·(kg·d) -1,连续用药 7d后断头采血 ,检测血肌酐 (Cr)、尿素氮 (BUN)、血栓素 B2 (TXB2 )和 6 酮 前列腺素F1α(6 keto PGF1α) ,并剖腹取其肾脏行常规病理检查。结果 :FK5 0 6 5mg·(kg·d) -1组血清BUN、Cr、TXB2明显高于空白对照组 (P均 <0 0 5 ) ,6 keto PGF1α较之显著降低 (P <0 0 5 ) ;光镜下可见肾近曲小管上皮细胞混浊肿胀 ,肾小球萎缩不明显。FK5 0 6 10mg·(kg·d) -1组血清BUN、TXB2明显高于FK5 0 6 5mg·(kg·d) -1组 (P <0 0 5 ) ,6 keto PGF1α显著降低 (P <0 0 5 ) ;光镜下可见肾小球萎缩 ,纤维化 ,严重者肾小球结构消失 ,肾近曲小管上皮细胞混浊肿胀。结论 :FK5 0 6的肾毒性主要表现为肾小球和肾小管损伤后的肾功能下降 。Aim:To study the nephrotoxicity and its mechanism of prograf(FK506).Methods:FK506 5 mg·kg -1 ·d -1 and 10 mg·kg -1 ·d -1 and physiological saline were injected into abdominal cavity of 3 groups of mice, repectively for 7 days, then the mice blood was taken for test of serum Cr?BUN?TXB2 and 6 keto PGF 1α ,and the kidneys were taken out for pathological examination Results:The levels of serum BUN,Cr and TXB2 in FK506 5mg·kg -1 ·d -1 group were obviously higher than those in control group( P <0 05),while 6 keto PGF 1α was markedly lower( P <0 05),and also proximal convoluted tubule epthelical cell presented swollen and turbid and no obviously atrophic glomeruli were observed.The levels of BUN?TXB2 in FK506 10 mg·kg -1 ·d -1 group were signficantly higher than those in FK506 5 mg·kg -1 ·d -1 group ( P <0 05),but 6 keto PGF 1α was lower ( P <0 05).Atrophic and fibrilized glomeruli were observed and in severe condition the glomeruli disppeared and epithelial cell became swollen.Conclusion: Nephrotoxicity of FK506 demonstrates as the renal dystemetion after the damage of glomeruli and tubule, and the mechanism is related with the injury of tubule epithelial cell.

关 键 词：普乐可复 肾脏毒性 肾移植 FK506 

分 类 号：R979.5[医药卫生—药品] R699.2[医药卫生—药学]