溶血等因素对血吸虫抗体检测影响的实验观察  

Effect of haemolysis on aetecition of antibody in schistosomiasis by indirect hemagglutination assay

作　　者：陈健康 肖祥[2] 

机构地区：[1]芜湖市第二人民医院,241000 [2]安徽省寄生虫病防治研究所

出　　处：《安徽预防医学杂志》1999年第4期374-375,共2页Anhui Journal of Preventive Medicine

摘　　要：本文着重讨论溶血、反复冻融、肝素抗凝对血吸虫抗体检测（ＩＨＡ 法） 的影响，结果表明：当标本中ＨＢ 含量＞ ２０ｇ／Ｌ 时，抗体滴度明显下降，在Ｈｂ 为１０ ～８０ｇ／Ｌ 范围内，抗体滴度与Ｈｂ 含量呈负相关；反复冻融３０ 次不影响抗体效价；肝素抗凝标本抗体效价较对照血清下降一个滴度，但与肝素含量无关。作者认为，以ＩＨＡ 法检测血吸虫抗体时，应避免标本溶血；The main aim of this article is to deal with whether haemolysis,serum treated by freezing and dissolving repeatedly and plasma prepared with heparin cna influence the antibody titer in schistosomiasis by IHA.The result showed haemolysis can decrease the antibody titer.When the concen tration of hemoglobin increased to 20g/L,the decrease become significant.Anegative correlation was found between antibody titer and the concentration of hemoglobin(10g/L—80g/L).No difference ,however,was found when serum was frizzed and dissolved for 0,5,15 and 30times.Antibody,titer was one times higher in control serum than that in all plasma specimes.It was suggested that haemolysis of specimes and plasma premredn with heparin should be avoided when we detect schistosome Antibody by IHA.

关 键 词：间接血凝试验 血吸虫病 IHA 抗体检测 临床诊断 

分 类 号：R532.21[医药卫生—内科学] R446.62[医药卫生—临床医学]