宫颈疾患中人乳头瘤病毒和疱疹病毒Ⅱ型DNA的检测  

Detection of Human Papillomavirus and Herpes Simplex Virus Type-2 DNA Sequences in Cervical Lesions

作　　者：孟祥金[1] 孙瑜[1] 刘知慧 陈敏诲[1] 李新志[1] 张有新[1] 李昆[2] 韩日才[2] 司静一 张卫[2] 曾维坤 胡伦颖[3] 农树桧[3] 

机构地区：[1]湖北医学院病毒研究所 [2]中国医学科学院基础所 [3]湖北医学院附二医院妇产科

出　　处：《病毒学杂志》1989年第1期22-27,共6页

摘　　要：本文应用HPV11,16,18型和HSV-2N/BglⅡ、HSV-2L/HindⅢDNA片段等五个分子探针,通过斑点杂交技术对79例宫颈疾患(包括50例宫颈癌和29例宫颈糜烂)组织DNA进行了检测,结果发现宫颈癌组织HPV16,18和11的阳性率分别为44％,12％和4％,而宫颈糜烂组织中HPV16,18和11的阳性率分别为14％,7％和14％;且3例标本HPV16和HPV18均呈弱杂交反应;在被检的所有宫颈癌组织中各有2例分别与HSV-2N/BglⅡHSV-2L/HindⅢ弱杂交,宫颈糜烂组织无一例阳性。结果提示,HPV在宫颈癌的发生过程中可能起主要作用,HSV-2的作用尚不确定,可能与HPV起协同作用。79 cervical lesions(50 cervical cancer and 29 cervical erosion) were studied by dot hybridization using five DNA probes: HPV 16, 18, 11 and HSV-2N/BglⅡ, HSV-2 L/HindⅢ. Among them, the positive percentage of HPV 16, 18 and 11 in cervical carcinoma was 44%, 12% and 4%, respectively; but 14%, 7%, and 14% in cervical erosion, respectively; and 3 samples showed weakly cross-hybridization between HPV 16 and HPV 18. of all cervical carcinoma studied, only 2 hybridize, weakly with HSV-2 N/BglⅡ and HSV-2 L/HindⅢ DNA respectively none were positive for HSV-2 in cervical erosion. These results indicate that HPV may play a major role in the development of cervical carcinoma, the possible effect of HSV-2 is unknown, it may have a synergism with HPV in the cervical cancer process.

关 键 词：人乳头瘤病毒 疱疹病毒Ⅱ型 宫颈病 

分 类 号：R466.5[医药卫生—临床医学]