阿魏酸钠对人类白细胞呼吸爆发氧代谢的影响  

Influence of sodium ferulate on human neutrophil-derived oxygen metabolites

作　　者：张兆辉[1] 余绍祖[1] 李庚山[1] 赵保路[2] 

机构地区：[1]武汉大学人民医院神经精神病研究所,武汉430060 [2]中国科学院生物物理研究所,北京100101

出　　处：《中国药理学通报》2001年第5期515-517,共3页Chinese Pharmacological Bulletin

基　　金：湖北省 1998年重点项目 ;No 981P160 2

摘　　要：目的　研究阿魏酸钠 (SF)对白细胞呼吸爆发期间氧消耗和过氧化物的影响。方法　采用电子自旋共振自旋捕集技术 ,自旋探针氧测定法和化学发光法检测白细胞呼吸爆发期间氧消耗和活性氧自由基。结果　SF( 1mmol·L-1)对白细胞呼吸爆发期间的氧消耗无影响 (P >0 0 5 ) ,但能抑制白细胞产生的化学发光反应 (P <0 0 1) ,清除白细胞释放的O·2 和·OH ,并在黄嘌呤 (Xan) /黄嘌呤氧化酶 (XO)体系和Fentons反应体系中证实 (P <0 0 1)。结论　SF不影响白细胞氧化代谢功能 ,能通过清除氧自由基防止激活白细胞对组织的损伤。AIM To investigate the influence of sodium ferulate (SF) on oxygen consumption during the respiratory burst of human neutrophils stimulated with PMA and superoxide. METHODS ESR spin trapping, spin probe oxymetry and luminol dependent chemiluminescence (CL) were used. RESULTS SF 1 mmol·L -1 had no influence on oxygen consumption during the respiratory burst of neutrophils ( P >0 05), but had remarkable inhibition on CL response generated by neutrophil ( P <0 01), and had scavenging effect on O · 2 and ·OH generated by neutrophils, which were demonstrated in Xan/XO system and Fentons reaction ( P <0 01). CONCLUSION SF has no inhibitory effect on oxygen metabolic function of neutrophils, but can prevent tissue injury caused by activated neutrophils through scavenging oxygen radicals.

关 键 词：阿魏酸钠 电子自旋共振 化学发光 氧自由基 抗氧化剂 白细胞 

分 类 号：R284.1[医药卫生—中药学]