白藜芦醇抑制HepG2细胞生长及诱导细胞凋亡的初步研究  

Prelimenary studies on effects of resveratrol in inhibiting growth of HepG2 and inducing its apoptosis

作　　者：田雪梅[1] 张展霞[1] 

机构地区：[1]中山大学化学与化工学院,广州510275

出　　处：《中国药理学通报》2001年第5期522-524,共3页Chinese Pharmacological Bulletin

摘　　要：目的　研究白藜芦醇 (Res)对人肝癌HepG2细胞生长增殖的影响 ,进而探讨Res诱导HepG2细胞凋亡的作用。方法　用不同浓度的Res处理HepG2细胞 ,MTT法检测Res对HepG2细胞生长增殖的抑制作用 ,通过HE染色、透射电子显微镜、原位末端标记法 (TUNEL)和流式细胞术观察凋亡细胞的形态结构变化以及定性、定量检测细胞凋亡。结果　Res能抑制HepG2细胞的生长增殖 ,并呈浓度依赖性 ;Res能诱导HepG2细胞凋亡。 结论　Res能够通过诱导HepG2细胞凋亡抑制HepG2细胞的增殖。AIM To investigate the effect of resveratrol on human liver cancer HepG2, and to study the function of resveratrol in inducing HepG2 apoptosis. METHODS HepG2 cells were treated with resveratrol at various concentrations. MTT assay was used to examine growth and proliferation of HepG2 cells after treatment with resveratrol for 24 hours. HE staining and electromicroscope were used to observe the morphology of apoptotic cells, and TUNEL, flow cytometry were also used to analyze the HepG2 apoptosis. RESULTS Resveratrol significantly inhibited growth and proliferation of HepG2 cells in dose dependent manner. And resveratrol also induced the apoptosis of HepG2 cells. CONCLUSION Resveratrol may inhibit HepG2 cell growth and proliferation through inducing apoptosis.

关 键 词：白藜芦醇 HEPG2细胞 细胞凋亡 心血管病 抗癌作用 防治 

分 类 号：R284.1[医药卫生—中药学] R285.5[医药卫生—中医学]