反相高效液相色谱法测定参附注射液及市售黑附片中乌头碱的含量  

Determination of aconitine in Shenfu injection and prepared aconite root by RP HPLC

作　　者：薛燕[1] 李农[1] 谢丽 

机构地区：[1]中国医学科学院.中国协和医科大学药物研究所,北京100050 [2]北京联合大学中医药学院,北京100013

出　　处：《中国药理学通报》2001年第5期585-586,共2页Chinese Pharmacological Bulletin

摘　　要：目的　测定参附注射液及市售黑附片中乌头碱的含量。方法　用RP HPLC ,流动相为乙腈 醋酸铵缓冲液 (pH10 5 ) ,紫外检测波长 2 3 0nm ,流速 0 8ml·min-1。结果　对照品的线性范围 1 3 9～ 2 2 2 4 μg(r =0 9997) ;市售黑附片中的乌头碱比参附注射液中的高 3倍。结论　方法简便、快速、准确、重现性好 。AIM To develop an assay for the determination of aconitine in Shenfu injection and prepared aconite root. METHODS RP HPLC was applied. The mobile phase consisted of acetonitrile ammonium acetate buffer solution (50∶50,pH 10 5),the detection wavelength was 230 nm and the flow rate was 0 8 ml·min -1 . RESULTS The linearity was obtained over the range of 1 39～22 24 μg( r =0 9997),aconitine concentration in the prepared aconite root was almost 3 times higher than that in the Shenfu injection. CONCLUSION The method is simple and fast, and can be used for the quantitative analysis of aconitine.

关 键 词：乌头碱 反相高效液相色谱法 附片 参附注射液 含量测定 

分 类 号：R284.1[医药卫生—中药学]