衍生化HPLC柱切换法测定人血浆中卡托普利浓度的方法研究  

Determination of Captopril in Human Plasma by HPLC with Derivatization and Column Swithing

作　　者：张群艳 李章万[1] 洪诤[1] 钱广生[1] 

机构地区：[1]华西医科大学药学院

出　　处：《药物分析杂志》2001年第4期273-276,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的 :建立高效液相色谱柱前衍生化结合柱切换技术测定血浆中卡托普利浓度的方法。方法 :血浆中卡托普利与对溴苯甲酰甲基溴发生衍生化反应后 ,分别加入等体积的丙酮和水 ,离心后取上清液进样 ,以HPLC柱切换进行分离测定。结果 :线性范围为 2 0～ 10 0 0ng·mL-1,净化回收率为 94 7%～ 96 2 % ,方法回收率为 98 1%～ 10 3 8% ,日内和日间检测RSD分别小于 2 8%和 4 2 %。结论 :方法简便灵敏 。Objective:To establish a method using HPLC with derivatization and column switching for determination of captopril(Cap) in human plasma.Methods:Cap in plasma was derivatized with p -bromophenacyl bromide ( p -BPB),then acetone and water were added into plasma with same volume,respectively.After centrifugalizing,the derivatization products,Cap- p \|BPB,in plasma samples were determined by HPLC with column switching.Results:A linearity range of 20～1 000 ng·mL\+\{-1\} was obtained.The clean-up recoveries were from 94 7% to 96 2%,and the method recoveries were 98 1%～103 8%.The RSD of within-day and between-day were less than 2 8% and 4 2%,respectively.Conclusion:The method is simple,sensitive and suitable for pharmacokinetics studies and clinical detection of Cap.

关 键 词：卡托普利 高效液相色谱法 巯基衍生 柱切换 高效液相色谱 

分 类 号：R972[医药卫生—药品] R969.1[医药卫生—药学]