新型无包膜DNA病毒恒河猴实验性感染的组织嗜性研究  

Tissue tropism of novel nonenveloped DNA virus in experimentally infected Rhesus monkey

作　　者：何海棠[1] 骆抗先[1] 肖红[1] 梁蔚芳[1] 杨守昌[1] 王燕军[1] 章廉[1] 

机构地区：[1]第一军医大学南方医院全军病毒性肝炎重点实验室,广州510515

出　　处：《中华医学杂志》2001年第14期871-875,共5页National Medical Journal of China

摘　　要：目的　探讨恒河猴实验感染新型无包膜DNA病毒 (TTV)的组织嗜性。方法　实验感染的恒河猴在病毒血症期间杀死 5只 ,取组织 ,用聚合酶链反应 (PCR)产物进行病毒半定量 ,并用原位杂交、负义探针斑点杂交和杂交 /核酸保护试验 ,检查复制型病毒DNA分子。结果　肝、外周血单个核细胞 (PBMC)和血清病毒含量 (电泳带密度 )依次为 5 0 1%、31 0 %和 18 9% ,相对比为 2 6 5∶1 6 4∶1 0 0。肝、脾、胃、小肠各段及其淋巴结、PBMC、结肠和血清均可检出病毒 ,但正链病毒DNA(假定的病毒复制中间体 ) ,只稳定存在于肝、脾、小肠和淋巴样细胞中。结论　这一病毒具肝、小肠和淋巴样细胞嗜性 ,可解释其多途径传播。Objective To investigate the tissue tropism of transfusion transmitted virus (TTV), a novel nonenveloped DNA virus, in experimentally infected Rhesus monkey. Methods Filtrate of feces of one patient with TTV infection was fed into the stomachs of ten Rhesus monkeys. Five (?) monkeys were killed during viremia and their tissues were collected to be examined for the presence of replicative virus DNA by in situ hybridization, dot blot hybridization with antisense probe and hybridization/nuclease protection assay. Results The virus contents (density of electrophoretic band) in the liver, PBMC, and serum of three infected monkeys 50.1%, 31.0%, and 18.9% respectively; the relative proportion was 2.65;1.64:1.00. TTV was detected in liver, spleen, stomach, small intestine and its lymph nodes, PBMC, colon, and serum; however, viral plus chain, a putative replicative intermediate, was found only in liver, spleen, small intestine, and lymphoid cells. Conclusion Transfusion transmitted virus is hepato , spleno , and lymphoid cell tropic, and may has multiple routes of transmission.

关 键 词：DNA病毒 肝 组织分布 无包膜DNA病毒 输血传播病毒 实验研究 

分 类 号：R512.6[医药卫生—内科学]