日本血吸虫重组BCG-Sj26GST疫苗免疫小鼠后IgG及其亚类和CAg的动态观察  

Dynamic observation on IgG、its subclasses and CAg elicited in mice immunized with recombinant BCG-Sj26GST vaccine of schistosoma japonicum

作　　者：李文桂[1] 石佑恩[2] 田音 周小林 汪娟 

机构地区：[1]重庆医科大学附属第一医院传染病寄生虫病研究所,400016 [2]同济医科大学寄生虫学教研室,武汉430030 [3]重庆市铜梁县人民医院,400322

出　　处：《重庆医学》2001年第6期481-483,共3页Chongqing medicine

基　　金：国务院总理基金资助项目 (94 Y 1 9)

摘　　要：目的　检测日本血吸虫重组BCG Sj2 6GST疫苗免疫小鼠不同时间后的血清IgG及其亚类和CAg反应。 方法　10 6CFU疫苗皮下和静脉注射免疫BALB/C鼠 ,分别于免疫后 0、4、8、10、14、16周各剖杀 4只 ,收集血清 ,常规ELISA法检测IgG及其亚类和CAg。结果　皮下注射组IgG在免疫后 14周 ,IgG1在免疫后 14～ 16周 ,IgG2a在免疫后 4周和IgG2b在免疫后 16周达最高水平 ,未能测到CAg ;静脉注射组IgG在免疫后 10周 ,IgG1和IgG2a在免疫后 8周以及IgG 2b在免疫后 16周达最高水平 ,CAg在免疫后 10周升高。结论　日本血吸虫重组BCG Sj2 6GST疫苗能诱导宿主产生高水平的IgG、IgG2a和IgG2b,静脉注射的免疫效果优于皮下注射。Objective To detect IgG、its subclasses and CAg after BALB/C mice were immunized by recombinant BCG Sj26GST vaccine of Schisotsoma japonicum at different time.Methods 4 BALB/C mice immunized subcutaneously and intravenously by 10 6 vaccine respectively were killed to gather sera on 0、4、8、10、14、16 weeks after immunization respectively,IgG、its subclasses and CAg were detected by routine ELISA.Results In the subcutaneous group,IgG on 10wk,IgG1 on 14～16wk,IgG2a on 4wk and IgG 2b on 16wk reached the highest level,but CAg could not be detected;in the intravenous group,IgG on 10wk,IgG1 and IgG 2a on 8wk and IgG2b on 16wk came up to the peak value,CAg increased on 14wk.Conclusion Recombinant BCG Sj26GST vaccine of schistosoma japonicum may induce host to produce high level of IgG、IgG2a and IgG2b,intravenous injection is a better route than subcutaneous injection when it's vaccinated.

关 键 词：日本血吸虫 重组BCG-Sj26GST疫苗 IgGIgGg亚类 CAG 

分 类 号：R392[医药卫生—免疫学]