皱纹盘鲍人工诱导雌核发育精子遗传失活的初步研究  

GENETIC INACTIVITY OF ABALONE SPERM BY UV FOR GYNOGENSIS

作　　者：张国范[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《海洋科学》2001年第10期37-39,共3页Marine Sciences

基　　金：国家自然科学基金资助项目39870700号

摘　　要：用波长253.7nm、光照强度1.108mW/cm2的不同剂量紫外线照射皱纹盘鲍 (HaliotisdiscushannaiIno)精子 ,然后与皱纹盘鲍的正常卵受精。在0～120s的照射范围内 ,受精作用都仍能正常发生 ,从早期胚胎存活率上可观察到“Hertwig”效应的存在 ,单倍体和非整倍体胚胎在孵化前死亡。在150000个精子/cm2的条件下 ,精液厚度5mm左右 ,经70s以上紫外线照射后 ,精子染色体可完全失活。The genetic inactivity of sperm in Pacific abalone Haliotis discus hannai Ino by UV in 253.7 nm wave length and a dosage of 1.108 mW/cm2 was investigated. The inactivity sperm was used to fertilize with normal eggs and the 'hertwig' effect was observed. The fertilization happened during 0±120 s radiation. The sperm radiated for 70 min would be inactive under the 150 000 sperm/cm2 with 5 mm thickness shaking in 100-150 time/min. Most of haploid and aneuploid embryos would not developed to the trochephore stage.

关 键 词：皱纹盘鲍 雌核发育 精子遗传失活 人工诱导 

分 类 号：S968.315[农业科学—水产养殖]