酵母双杂交系统及其应用  

Yeast two-hybrid system and its applications

作　　者：张晓光[1] 药立波[1] 苏成芝[1] 

机构地区：[1]第四军医大学生物化学与分子生物学教研室,西安710032

出　　处：《生命科学》2001年第5期228-231,共4页Chinese Bulletin of Life Sciences

摘　　要：酵母双杂交系统（ｙｅａｓｔ ｔｗｏ－ｈｙｂｒｉｄ　ｓｙｓｔｅｍ）是直接于细胞内研究蛋白质间相互作用的一种灵敏度很高，且非常有效的遗传学方法，在不同研究领域中广泛应用，并不断完善及改进，发展出单杂交系统（ｏｎｅ－ｈｙｂｉｄ ｓｙｓｔｅｍ） 和三杂交系统 （ｔｈｒｅｅ－ｈｙｂｒｉｄ ｓｙｓｔｅｍ）等一系列相关的技术，克服了原系统的局限。本文对双杂交系统的原理、发展概况、应用、存在问题和前景等进行了综述。The yeast two-hybrid system is a quickly developing genetic assay which provides a highly sensitive and efficient genetic means to detect protein-protein interaction in vivo. It have witnessed abroad application as well as modification and permutation of the basic two- hybrid system. Such improvements as the one-and three-hybrid systems and some other related techniques promise to overcome some limitations of original system. We review the principle, development, application, some questions and future prospect of two-hybrid system.

关 键 词：酵母双杂交系统 蛋白-蛋白相互作用 筛选 应用 

分 类 号：Q75[生物学—分子生物学] Q51